UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 8 / No. 3 / Published : 2015-11

Order : 2, and page :245 - 250

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Original Article :

Effect of insulin transferrin selenium (its) supplementation in maturation medium tcm-199 to bovine oocyte diameter

Author :

  1. Reza Mahendra Yudha Permana*1
  2. Widjiati*2
  3. Budi Utomo*3
  4. Tjuk Imam Restiadi*4
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan
  4. Dosen Fakultas Kedokteran Hewan

Abstract :

Insulin Transferrin Selenium (ITS) has been used in vitro maturation system to support in vitro maturation of oocyte. The study aims was to determine role of Insulin Transferrin Selenium to bovine oocyte were matured in vitro against improved oocyte diameter. Maturation process used TCM-199 medium which added by FSH 100 IU/ml, LH 100 IU/ml, BSA 3% and ITS (Insulin Transferrin Selenium). ITS dose which used in culture medium are different for each treated group. The treated groups are P0 (0 μg/ml), P1 (10 μg/ml), P2 (15 μg/ml), and P3 (20 μg/ml). Oocytes were incubated in CO2 incubator with CO2 5%, Humidity 95% and 38.5oC due 22 hours. The result showed that ITS supplementation inmaturation medium has no significantly difference (p>0,05) for all treated groups (P0, P1, P2, and P3) of oocyte diameter improvement. Conclusion of ITS supplementation in maturation medium TCM-199 can’t increase oocyte diameter.

Keyword :

oocyte, in vitro maturation, Insulin Transferrin Selenium, diameter,


References :

Widjiati, D. Wulansari, Wurlina, dan N.M.R Widjaja.,(2011) Identifikasi Growth Differentiation Factor (GDF-9) dari Maturasi In Vitro Oosit Sapi Dengan Teknik Imunositokimia 5 (2): 59-62 : Jurnal Kedokteran Hewan

Liu, X., J. Liu, N. Kang, L. Yan, Q. Wang. X. Fu, Y. Zang, R. Xiao, and Y. Cao,(2014) Role of Insulin-Transferrin-Selenium in Auricular Chondrocyte Proliferation and Engineered Cartilage Formation in Vitro 15: 1525-1537 : International Journal of Molecular Science

Daoed, D.M., N. Ngadiyono dan D.T. Widayati,(2013) Effect of Fetal Calf Serum Supplementation on In Vitro Maturation Ability of Bovine Oocytes 37 (3): 136-142 : Buletin Peternakan





Archive Article

Cover Media Content

Volume : 8 / No. : 3 / Pub. : 2015-11
  1. Genotyping Viral Nervous Necrosis (vnn) In Groupers In East Java And West Nusa Tenggara
  2. Lactoferrin Effect On Biofilm Formation Of Klebsiella Pneumoniae Isolated From Mastitis Cases Of Dairy Cows
  3. Effect Of Insulin Transferrin Selenium (its) Supplementation In Maturation Medium Tcm-199 To Bovine Oocyte Diameter
  4. Supplementation Of Insulin Transferrin Selenium On In Vitro Maturation Cumulus Oocyte Complex (coc) Against To Mitogen Activated Protein Kinase (mapk) Expression And Expanded Cumulus Mass
  5. Adaptation Capability And Antigen Stability Of Newcastle Disease Virus Local Isolate On Hela Cell
  6. Comparison Of Antibody Titre Of Anti Brucella Abortus Using Cft And Ielisa To Cattle With Positive Rbt
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  8. Suplementation Of Specific Protein Membrane Spermatozoa In Freezing Medium To Improvement Efforts A Succes Cattle Frozen Semen Quality Post Thawing
  9. Potention Of Alkaloid Sambiloto (andrographis Paniculata L) To Total Leukocytes And Leukocytes Differential Count On Rat After Infected By Salmonella Typhimurium
  10. The Potential Of Outer Membrane Protein Brucella Abortus Local Isolate As Brucellosis Vaccine Development In Indonesia
  11. The Using Of Whole Serum Pmsg (pregnat Mare Serum Gonadotropin) With Sepadex Extracted For Increasing Estrus Induction Of Ettawa Goat
  12. Toxicity Test Of Singawalang (petiveria Alliaceae) Leaves Extract To The Hypertrophy Of Male White Mice (mus Musculus) Cardiac Muscle
  13. Effect Of Benzo(a)pyrene Injection To The Initiation Of Mammae Cancer At Mice

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