UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 9 / No. 3 / Published : 2016-11

Order : 6, and page :37 - 44

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Original Article :

Effect of moringa oleifera leaf extract on the percentage of necrotic purkinje cells in mice (mus musculus) induced by methylmercury

Author :

  1. Annisa Karimah*1
  2. Nunuk Dyah Retno Lastuti*2
  3. Suryo Kuncorojakti*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

Penelitian ini bertujuan untuk mengamati pengaruh pemberian ekstrak daunMoringa oleiferaterhadap presentase nekrosis sel Purkinje pada cerebellum mencit(Mus musculus) yang dipapar metilmerkuri. Sejumlah 20 mencit jantan digunakanpada penelitian ini dan kemudian dibagi menjadi lima kelompok (C –, C +, T 1, T 2,T 3). C – diberi 0.2 ml CMC Na, C + diberi 0.4 mg/kg bb metilmerkuri, T 1 diberi 200mg/kg bb ekstrak daunMoringa oleifera + 0.4 mg/kg bb metilmerkuri, T 2 diberi 400mg/kg bb ekstrak daun Moringa oleifera + 0.4 mg/kg bb metilmerkuri, dan T 3 diberi800 mg/kg bb ekstrak daun Moringa oleifera + 0.4 mg/kg bb metilmerkuri. Perlakuanterhadap kelompok percobaan dilakukan selama 21 hari, kemudian mencit dikorbankandengan metode dislokasi servikalis. Cerebellum diambil dan dijadikan preparat histologi.Pengamatan mikroskopik dilakukan untuk menghitung jumlah total sel Purkinje dansel Purkinje nekrosis. Hasil penelitian menunjukkan bahwa ekstrak daun Moringaoleiferamenurunkan presentase nekrosis sel Purkinje pada cerebellum mencit (Musmusculus) yang dipapar metilmerkuri. Ekstrak daun Moringa oleiferadengan dosis 800mg/kg bb memberikan efek protektif yang paling baik dibandingkan dengan dosis 200dan 400 mg/kg bb.

Keyword :

Moringa oleifera, Metilmerkuri, Cerebellum, Sel Purkinje,


References :

Broussard, L.A., C.A Hammet-Stabler, R.E Winecker and J.D Ropero- Miller. ,(2002) The Toxicology of Mercury. 33:614-625. : Laboratory Medicine.

Luqman, S. S. Srivastava, R. Kumar, A. K. Maurya and D. Chanda. ,(2011) Experimental Assesment of Moringa oleifera Leaf and Fruit for Its Antistress, Antioxidant and Scavenging Potential Using In Vitro and In Vivo Assays. 2012:1- 12. : Evidence-Based Complementary and Alternative Medicine.

Newland, M.C. ,(2002) Neural-behavior Toxicity of Methylmercury andPCBs Effects-profiles and Sensitive Populations. 12: 119-128. : Environmental Toxicology and Pharmacology.





Archive Article

Cover Media Content

Volume : 9 / No. : 3 / Pub. : 2016-11
  1. The Effect Of Spirulina Platensis Extract Immersion As Immunostimulant To Intestine Histopathology Of Aeromonas Hydrophila Infected Gouramy (osphronemus Gouramy)
  2. Effect Of Vitamin E And Vitamin C Combination As Preventive Measure On The Leydig Cell Number In Mice (mus Musculus) Treated With Borax
  3. The Influence Of Beta Carotene To Live Fetus Percentage In Mice (mus Musculus) That Exposured By Cigarette Smoke
  4. Effects Of Vitamin C And Vitamin E Combinationas Prevention On The Liver Histopathological In Mice (mus Musculus) Treated With Borax
  5. Effect Of Moringa Oleifera Leaf Extract On The Percentage Of Necrotic Purkinje Cells In Mice (mus Musculus) Induced By Methylmercury
  6. Hepatoprotective Effect Of Andrographis Paniculata Ness. Leaves Extract Towards Mice (mus Musculus L.) Liver Histopathology Which Exposured Bylead Acetate Orally
  7. The Effect Of Mimosa Pudica Root Extract To Histopathological Representation Of Rattus Norvegicus Liver Injected With Naja Sputatrix Venom
  8. Kualitas Spermatozoa Domba Merino Pada Sisi Anoda Hasil Pemisahan Dengan Teknik Ess ( Electric Separating Sperm)
  9. Effectivity Of Rat Bone Marrow Mesenchymal Stem Cell Therapy On Rattus Norvegicus Exposed To Carbon Black As Observed From Caspase-3 Expression
  10. Larvaside Effectiveness Of Srikaya Seed Extract (annona Squamosa L.) Against Mortality Of Anopheles Aconitus Larvae
  11. Therapeutic Effect Rat Bone Marrow Mesenchymal Stem Cell (rbm-msc) At The White Rat Endothelial Cells Induced Carbon Black To Total Hofbauer Cells
  12. Identification Of Bacteri Methicillin Resistent Staphylococcus Aureus And Methicillin Sensitive Staphylococcus Aureus From Dog Nasal Mucous
  13. Expression Of Cytochrome C And Capacitation Reaction Frozen Semen Fresian Holstein Bulls (fh) After Thawing In Different Temperature And Duration In Diluent Milk Egg Yolk
  14. Detection Of Bacterial Contamination Amount At Meat Which Is Soaked In Chitosan Solution With Total Plate Count Method
  15. The Effect Of Electrical Stunning On The Ph Values Musculus Fibularis Longus Broiler Chicken

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