UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 7 / No. 1 / Published : 2014-02

Order : 7, and page :43 - 48

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Original Article :

Effect of seminal plasm of cow on goat’s spermatozoa plasma membran in milk diluter on semen freezhing

Author :

  1. Suherni Susilowati*1
  1. Dosen Fakultas Kedokteran Hewan

Abstract :

Up till now semen freezing process of ram’s spermatozoa has not yet satisfying, this is because ram’s seminal plasma contain phospholipase A from bulbourethral gland, that can coagulate with egg yolk in diluter. Because of that researchers want to replace ram’s seminal plasma with bull’s seminal plasma in milk diluter media. Special aim of this research is to understand the benefit of bull’s seminal plasma in diluter of ram’s spermatozoa. General purpose of this research is using bull’s seminal plasma in diluter of ram’s spermatozoa to improve fresh semen quality and even frozen semen. This research consists of three treatments. Control treatment (PO) are diluter + ram’s semen (without seminal plasma). Treatment I (PI) are diluter + spermatozoa + bull’s seminal plasma (1:1), treatment II (PII) are diluter + spermatozoa + bull’s seminal plasma (1:2). All of treatments observed everyday of motility, viability, plasma membran integrity and spermatozoa’s DNA integrity. Observation in diluter without seminal plasma shows lowest spermatozoa’s membrane plasma integrity in day I, II, III, IV, and V. Anova test for spermatozoa’s membrane plasma integrity shows significant different (p <0,05) between PO, PI, PII, and PIII. Duncan's multiple range test for observation PI group produce highest spermatozoa’s membrane plasma integrity percentage.In conclusion, addition of bull’s seminal plasma can mantain, membrane plasma integrity in skim milk diluter

Keyword :

Seminal plasma, Bull, ram’s, Membran plasma integrity,


References :

Beatriz, B, Perez-Pe, A. Gallego Tato, J. Osada, T. Muino Blanco and J.A.Cebrian Perez,(2000) Seminal Plasma Protein Revert the Cold Shock Damage on Ram Sperm Membrane . : Biology Reproduction. 63:1531-1537

Lamirande, E.H., A. Jiang, H. Kodana, and C. Gagnon,(1997) Reactive Oxygen Species and Sperm Physiology . : Rev.Reprod. 2(1) :48-54

Ramukhiti, F, L. N. Tshimangadzo, B. Sutherland and C.L. Khoboso,(2011) Cryopreservation of South African indgenous goat semen. African Journal of Biotechnology Africa : African Journal of Biotechnology. 10 (77): 17898-17901





Archive Article

Cover Media Content

Volume : 7 / No. : 1 / Pub. : 2014-02
  1. Grant Of Ethanol Extract Of Seeds Papaya (carica Papaya) In Female Rats (rattus Novergicus) Antifertilitas Material, As An Alternative To Number And Quality Of Egg Cells
  2. Effect Of Infusum Salam Leaves (eugenia Polyantha) On Blood Glucose Level On The Rat (rattus Norwegicus) In Alloxan Induced
  3. Potential Of Phytase Ezymes From Rumen Bacteria On Sem Analysis Structural Changes Of Rice Bran As Broiler Feed
  4. The Comparison Of Morphology Between Bovine Oocyte Vitrification Before And After In Vitro Maturation
  5. The Effect Of Exposing Pregnant Rat (rattus Novergicus) To Particulate Matter On Congenital Disorder And Resorption Of Embryos
  6. Ha Gene Phyloanalysis Of Influenza H1n1 2009 Virus In Surabaya
  7. Effect Of Seminal Plasm Of Cow On Goat’s Spermatozoa Plasma Membran In Milk Diluter On Semen Freezhing
  8. Histopathological View Pyramid Cell On Cerebrum Of White Rat (rattus Norvegicus) Were Exposed With Methylmercury Which Administrated With Cdp-choline As Neuroprotective Agent
  9. Giving Epigallocathechin Gallate (egcg) Toward The Expression Of Estrogen Receptor At Female White Mouse (rattus Norvegicus)
  10. Human Resource Management Goad Farm Of The Cross Breed Etawa Goat Independent Farm, Farmer Groups And Businesses In The Town Of Turi Sleman Regency
  11. The Pattern Of Antimicrobial Susceptibility Of Salmonella Sp Isolated From Beef
  12. Potential Mycotoxin Binders On Histopathology Of The Mammary Gland Experiencing Neoplasia In Pregnant Mice (mus Musculus) With Exposed Zearalenone
  13. Potential Of Mycotoxin Binders On Weight And Length Of Fetus Pregnant Mice (mus Musculus) With Zearalenone Exposure
  14. Prevalence Of Worm Disease In Cattle Gastrointestinal Peranakan Ongole (po) And Simental Cattle In The Subdistrict Laren, District Lamongan
  15. The Giving Of Anti- Toxoplasma Gondii Membran Immunoglobulin Y Before Infection Reduce Apoptotic Index Liver Mice

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