Veterinaria Medika
ISSN 1979-1305
Vol. 2 / No. 1 / Published : 2009-02
Order : 11, and page :55 - 60
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Original Article :
Identification of encoding gene for fibrinogen binding protein on staphylococcus aureus in mastitic milk
Author :
- Mustofa Helmi Effendi*1
- Fakultas Kedokteran Hewan Universitas Airlangga
Abstract :
The experiment to be done to show identification of encoding gene for bacterial fibrinogen binding protein on Staphylococcus aureus. The first step of test was to prepare pure culture of Staphylococcus aureus. Milk samples were collected from mastitic cases at the afternoon milking time. Preparation of pureculture were confirmed by MS agar, hemolytic activity, catalase test and coagulase.The result showed that there was a encoding gene of bacterial fibrinogen binding protein of Staphylococcus aureus with molecular size 1000 bp. The characterization molecular weight of bacterial fibrinogen binding protein was 40 kD.
Keyword :
Encoding Gene, Fibrinogen Binding Protein, Staphylococcus aureus, ., .,
References :
Deivayagam, C.C.S., E.R. Wann, W. Chen, M. Carson, and V.L. Narayana,(2002) A Novel Variant of the Immunoglobulin Fold in Surface Adhesins of Staphylococcus aureus: Crystal structure of the Fibrinogen-Binding MSCRAMM, Clumping Factor United States : The EMBO J.
Cunninghan, R., A. Cockayne and H. Humphreys,(1996) Clinical and Molecular Aspects of the Patogenesis of Staphylococcus aureus bone and joint infections United States : J. Med. Microbiol
Akineden, O., C. Annemuller, A.A. Hassan, C. Laemler, W..Wolter and M. Zschok,(2001) Toxin Genes and Other Characeristics of Staphylococcus aureus Isolates from Milk Cows with Mastitis United States : Clinical and Diagnostic Laboratory Immunology
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