UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 3 / No. 3 / Published : 2010-11

Order : 10, and page :201 - 204

Related with : Scholar   Yahoo!   Bing

Original Article :

Identification of kit-ligand from in vitro maturation of bovine oocytes with electrophoresis method

Author :

  1. Widjiati*1
  2. Yusak Beato W.P*2
  3. Chairul Anwar*3
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The expression patterns of Kit-Ligand and c-kit in the ovary, as well as the actions of Kit-Ligand on oocytes and theca cells, suggest that Kit-Ligand important for many stages of follicular development in the ovary. The aim of this experiment was to identify and characterize the Kit-Ligand protein fraction that isolated from mature oocytes of bovine ovaries obtained from all folikel. The folikel of bovine ovaries obtained from slaughterhouse were aspired. The oocytes were matured in Tissue Culture Medium 199 (TCM 199) then culture for 22 hours at 38,5 ºC in incubator CO2. Identification of Kit-Ligand protein fraction was performed with Sodium Dodecyl Sulphate Polyacrilamid Gel Electrophoresis (SDS-PAGE) process. From the result of this experiment, saveral protein band have appeared. Based of the calculation using regression equation on the protein marker for estimating the molecular weight of Kit-Ligand ,12 protein fractions were obtained : 193,09 kDa, 172,51 kDa, 105,84 kDa, 89,37 kDa, 64,94 kDa, 53,81 kDa, 39,84 kDa, 36,27 kDa, 30,63 kDa, 26,35 kDa, 22,68 kDa, 20,64 kDa. Protein appearing in the protein band the molecular weight of 36,27 kDa was identified as protein Kit-Ligand that has the important role in the follicle development.

Keyword :

Kit-Ligand, oocyte, follicle, bovine ovary, SDS-PAGE,


References :

Affandhy, Lukman, W. C. Pratiwi, Ratnawati dan Dian,(2007) Petunjuk Teknis Penanganan Gangguan Reproduksi Pada Sapi Potong Grati : Pusat Penelitian dan Pengembangan Peternakan Badan Penelitian dan Pengembangan Peternakan Departemen Pertanian

Aulanni’am,(2005) Protein dan Analisisnya Malang : Citra Mentari Group

Nilsson, Eric, Roger, Natalie and M. K. Skinner,(2007) Actions of anti-mullerian hormone of on the ovarian transcriptome to inhibit primordial to primary follicle transition. USA: Center for Reproductive Biology Pullman-USA : School of Moleculer Biosciences. Washington State University





Archive Article

Cover Media Content

Volume : 3 / No. : 3 / Pub. : 2010-11
  1. Garlic As An Alternative Disinfectant To The Escherichia Coli
  2. Centrifugation Of Goat Semen At Freezing Process With Some Concentrate Of Egg Yolk Diluent To The Quality Of Sperm
  3. Influence Of The Avian Influenza Virus Subtype H5n1 Infection Toward Sperm Motility And Life In Long Tail Monkey (macaca Fascicularis)
  4. Vfa Concentration And Acetate, Propionate, Butyrate Molar Proporsions Rumen Of Ongole Grade Cattle With Ammoniation Rice Straw, Soybean Straw And Rice Straw
  5. The Activity Of Achyranthes Aspera Linn Alkaloid To Cause Aopoptotic And Dna Fragmentation On Breast Cancer
  6. The Antimitogenic Effect Of Alkaloid Achyranthes Aspera Linn On Apoptotic Induction In Cell Infection By Mycobacterium Tuberculosis
  7. Stages Of Vaccination And The Type Of Vaccine Used To Prevent Infectious Disease In Chickens
  8. Effect Of Complex Of Insulin Like Growth Factor-1 And Insulin Like Growth Factor Binding Protein-3 On The Increase Number Of Ovarian Follicle In Female Mice (mus Musculus)
  9. Spleen Weight And White Pulp Diameter Picture In Broiler Chickens Exposed To Cronic Heat Stress
  10. Identification Of Kit-ligand From In Vitro Maturation Of Bovine Oocytes With Electrophoresis Method
  11. Cellulolityc Bacteria For Increasing Quality Of Complete Feed From Waste Product
  12. Identification Of Cyclin-dependent Kinase (cdk1) That Express On Bovine Cumulus Oocyte Complex After A Model Oocyte Maturation Experimence
  13. Utilization Of Bakteriocin In The Handling Of Sub Clinical Mastitis And The Effect On Milk Quality On The Number Of Bacteria
  14. Expression Of Adhf-36kda Protein Towards Osmolarity And Ph In Vitro Environmental Of Salmonella Typhi (as Study To Adhesin Molecule-based Candidate Vaccine For Typhoid Fever)
  15. Motility And Live Percentage Test In Ram’s Spermatozoa In Isotonic Commercial Liquid And Egg Yolk Assortment Extender
  16. Identification Of The Gene For Staphylococcus Aureus Toxic Shock Syndrome Toxin-1 (tsst-1) From Bovine Mastitis Milk

Information index.php/JIPK/VETMED