UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 1 / No. 1 / Published : 2008-02

Order : 14, and page :67 - 72

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Original Article :

Influence of leaf extract of sirih ( piper betle l.) to growth staphylococcus aureus and escherichia coli with disk diffusion method

Author :

  1. Anang Hermawan*1
  2. Hana Eliyani*2
  3. Wiwik Tyasningsih*3
  1. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  2. Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

Bakterisidal effect had been known in sirih plant ( Piper betle L.). This research was to know bakterisidal effectand the effectivity of leaf extract of sirih ( Piper Betle L.) based on in-vitro test to bacteria of Staphylococcusaureus and Escherichia coli. This Research had used diffusion methode that sensitivity knowledgeable Kirby-Bauer test with five treatment and seven times restating. Five treatment were consisted of negatif control 10 %DMSO, leaf extract of sirih was 2,5; 5, 10% and antibiotic as positif control. Paper disk sterile soaked in 10%DMSO and in leaf extract of sirih with concentration of 2,5; 5 and 10% respectively. Paper disk have beensoaked to be evaporated in incubator till the disk to dry, then put down at media Mueller Hinton Agar that hasbeen inoculated Staphylococcus aureus with oxacillin and also Escherichia coli with sulfonamide, then incubationat temperature of 37°C for 24 hours. The result of research shown at concentration 2,5; 5 and 10% ofleaf extract of sirih and also antibiotic can inhibited growth of Staphylococcus aureus and Escherichia coli.With double distance test of Duncan indicate that highest result of antibacterial effect of leaf extract of sirih toStaphylococcus aureus is by concentration at 10% that not different significant with concentration of 2,5 and5%, while antibiotic of Oxacillin show lower result than third concentration of leaf extract of sirih. Highestresult Escherichia coli shown by sulphonamide antibiotic, comparated with third concentration of leaf extractof sirih. Control 10% DMSO don’t have effect to both bacteria.

Keyword :

Extract leaf of sirih, Staphylococcus aureus bacteria, Escherichia coli bacteria, Disk diffusion method, .,


References :

Biesher,(1983) Microbiology in Practice. Individualized Introduction for The Allied Heath Science. 3rd ed New York : Harper and Row Publisher

Breed, R.S., E.G.D Murray and N.R Smith,(1957) Bergey’s Manual of Determinative Bacteriology. 7th ed. . : Williams and Walkins





Archive Article

Cover Media Content

Volume : 1 / No. : 1 / Pub. : 2008-02
  1. Identification Of Staphylococcus Aureus Which Causes Mastitis With Mannitol Test And Detection Of Acetoin Product In Dairy Cattke At The Farm Area Of Suka Makmur Dairy Cooperative Grati Pasuruan
  2. The Influence Of Api - Api (avicennia Marina) Leaves Extract Toward Embryo Resorbtion, Body Weight And Lengh Toetal Mice (mus Musculus)
  3. The Effects Of Eimeria Tenella Infection On The Spleen Weight, Size And White Pulp Diameters In The Chickens Primary Infection And Secondary Infection
  4. Detection Of Avian Influenza Virus Subtype H5 On Stray Cats (felis Silvestris Catus) In Bandung City Area
  5. The Effect Of Alkaloid Fraction Of Jarong (achyrantes Aspera Linn) On Viability Culture Myeloma Cell Mice
  6. Antibacterial Activity Of The Ethanol Extract Pluchea Indica Less Leaves Against Escherichia Coli By In Vitro
  7. Identification Of Protein Profile Of Sarcoptes Scabiei On Goats By Sds-page Analysis
  8. Identification Of Excretion-secretion Protein Profile Of The Adult Haemonchus Contortus With Sds-page
  9. Identification Of Aerob Cellulolytic Fungi From Rumen Fluid Waste At Pegirian Slaughter House Surabaya
  10. The Effects Of Supplying Commercial Probiotic On The Quantity And Quality Of Dairy Cow Milk
  11. The Effect Of Given Crude Chlorella To Total Cholesterol Blood Rates On Broiler Chicken
  12. The Effect Sgot And Sgpt Level Of Whiterat ( Rattus Norvegicus ) That Was Given Juice Of Noni ( Morinda Citrifolia ) And High Fatty Diet
  13. Utility Information Technology ( It ) For Head Surface Area Of Spermatozoa Sheep With Biometric Measurement Methode
  14. Influence Of Leaf Extract Of Sirih ( Piper Betle L.) To Growth Staphylococcus Aureus And Escherichia Coli With Disk Diffusion Method
  15. Protein Characterization Of Excretory Secretory Second Stage Larvae Dorman Against Antibody Anti-second Stage Larva Toxocara Canis By Western Blot Techniques

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