UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 4 / No. 2 / Published : 2011-07

Order : 7, and page :115 - 120

Related with : Scholar   Yahoo!   Bing

Original Article :

Isolation identification and sensitivity test of bacillus subtilis from shrimp culture ponds to antimicrobial agents

Author :

  1. Rosilawati, Erni*1
  2. Christinasari, Y.C*2
  3. Koestanti, Emy*3
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The aim of this study was to know about the characteristic and the sensitivity of Bacillus subtilis isolated from shrimp culture ponds to antimicrobial agents. Fifty isolates of Bacillus spp. That fermented mannitol on MSA media and showed rod shapes, had been identified by macroscopic, microscopic, biochemical and sugars test. The result showed six isolates were Bacillus subtilis. Antimicrobial agents thathad been used for sensitivity test were Penicillin, Ampicillin, Oxytetracycline, Gentamicin, Ciprofloxacin,and Sulfamethoxazole by in vitro using Kirby- Bauer method. Disc of these antimicrobial agents were placedon the cultures of Bacillus subtilis at the surface of MHA media, incubated at 37 C for 24 hours. Diameter of inhibitor area around the disc was measured on mm to identify the isolates resistant or sensitive. The sensitivity test result that Bacillus subtilis sensitive to all of the antimicrobial agents with range of diameter inhibition was 16 until 38 mm. It means that Bacillus subtilis didn’t have gen resistant and couldn’t transfer it to pathogen bacteria in shrimp culture so that it was very safe for use as a probiotic. It was suggested to do another identification test to make sure about Bacillus subtilis and do in vivo study to know the isolates safe to be used as a probiotic or not 

Keyword :

Bacillus subtilis, Sensitivity test, Antimicrobial agents,


References :

Balcazar, J.L. and T. Rojas-Luna.,(2007) Inhibitory Activity pf Probiotic Bacillus subtilis UTM 126 Against Vibrio Species Confers Protection Against Vibriosis in Juvenile Shrimp (Litopenaeus vannamei) United States : Curr. Microbiol. 55: 409-412.

Cappucino, J.G. and N.Sherman.,(2005) Microbiology: A Laboratory Manual. 7 th edition United States : Pearson Education Inc.

Adewumi, G.A., R.A. Quadri, and F.A. Oguntoyinbo,(2009) Antibiotic Sensitivity Pattern of Bacillus Species Isolated from Solid Substrate Fermentation of Cassava for Gari Production Africa : African Journal of Microbiology Research 3:11.





Archive Article

Cover Media Content

Volume : 4 / No. : 2 / Pub. : 2011-07
  1. The Effect Of Tyrosine Kinases Addition Into The Egg Yolk Skim And Egg Yolk Tris Extender The Motility And Live Spermatozoa Percentages Of The Holstein Friesian Post Thawing
  2. Avian Influenza H5 Virus Excretion Post Challenge Test Of Chickens Immunized With Avian Influenza (h5n1) Virus Indonesian Strain And Commercial Heterologous Vaccine
  3. Reepithelialization And Ck 16 Expression On Superficial-thickness Wound With Topical Hyaluronate And Freeze-dried Amniotic Membrane Treatment
  4. The Effects Of Various Concentration Propanediol Cryoprotectan At Vitrification Process To Viability Of Mice Embryos Post Thawing
  5. Activity Test Of Gynura Procumbens Leaves Extract As Antiangiogenic On Chick Embryo Chorioallantoic Membrane Induced By Basic Fibroblast Growth Factor (bfgf)
  6. Efect Of Centrifugation Of Goat Semen To Free Radical Concentrate On Washing Medium
  7. Isolation Identification And Sensitivity Test Of Bacillus Subtilis From Shrimp Culture Ponds To Antimicrobial Agents
  8. The Differences In Several Parameters Of The Femur Bone Related To The Biomechanics Of The Bucks And Doe
  9. The Influence Of Temperature On Meat Decay One Of Beef Sold The Traditional Market
  10. Antibody Response And Protectivity In Chickens After Vaccination With Nd Lv12 Active Vaccine
  11. Concentration Of Pregnancy Associated Glycoprotein (pag) Of Milk Of Dairy Cows At 21 And 28 Days Post Artificial Insemination
  12. The Increase Of Activated Goblet Cells In The Duodenum Of Broiler Exposed By Heat Stress
  13. (z)-9- Heptacosene Sex Pheromone Level Production On Cuticular Hydrocarbon Field Of Strain Musca Domestica
  14. Antibacterial Potency Of Honey And Noni Juice (morinda Citrifolia L.) To The Number Of Staphylococcus Aureus On Chicken Carcasses
  15. Mengukur Kadar Protein Fertility Associated Antigen (faa) Pada Semen Sapi Menggunakan Qubit Fluorometer

Information index.php/JIPK/VETMED