UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 8 / No. 2 / Published : 2015-07

Order : 14, and page :107 - 214

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Original Article :

Isolation of methicillin-resistant staphylococcus aureus (mrsa) in dog nasal swab with oxacillin screen agar confirmation test

Author :

  1. Nanis Nurhidayah*1
  2. Mustofa Helmi Effendi*2
  3. Nenny Harijani*3
  4. Fedik Abdul Rantam*4
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan
  4. Dosen Fakultas Kedokteran Hewan

Abstract :

This study aims to isolate, identificate and confirm the presence or existence of Methicillin-Resistant Staphylococcus aureus (MRSA) in dog nasal swab as samples. The research was conducted at Laboratory of Veterinary Bacteriology and Mycology, and Laboratory of Veterinary Public Health, Airlangga University from September until November 2013. Samples were taken randomly from 30 dogs in Surabaya. The research showed 16 (53%) specimens growth in MSA (fermented mannitol). Gram staining and microscopic examination resulted 12 (40%) specimens colonies identified as coccus and 4 (13%) specimens were bacil shapes colonies. The result of hemolysis test using Blood Agar Medium; 3 (10%) specimens were beta hemolysis (specimens number 9, 12 and 15). This three samples were tested with antibiotics and susceptibility testing Kirby-Bauer Disk diffusion method. All samples were resistant to erythromycin (100%), penicillin (100%) and methicillin (100%). The research was completely confirmed the growth of MRSA from specimens number 9 and 12 in oxacillin screen agar medium, named Poci (5 months) and Moli (5 years). Both are dogs from different breeder in Kutisari, South Surabaya.

Keyword :

MRSA, Oxacillin Screen Agar, dog,


References :

Khan SA, M. S. Nawez, A.A. Khan, and Cerniglia,(2000) Transfer of erythromycin resistance from poultry to human clinical strain of S. aureus 38: 1832 : J. Clin. Mricrobiol

Saga, T. and K. Yamaghuchi,(2009) History of Antimicrobial Agents and Resistant Bacteria 52(2): 103–108 : Journal of Medicine Association Journal

Nicola, F. G., L. K. Mcdougal, J. W. Biddle, and F. C. Tenover,(1998) Characterization of Erythromycin-Resistant Isolates of Staphylococcus aureus Recovered in the United States from 1958 through 1969 42(11): 3024-3027 : Antimicrobial Agents and Chemotherapy





Archive Article

Cover Media Content

Volume : 8 / No. : 2 / Pub. : 2015-07
  1. The Effect Of Acupuncture On Sodium, Potassium, And Chloride Blood Plasma In Thoroughbred Horse After Exercise
  2. The Effects Of Insulin Transferin Selenium (its) Supplementation On Embryo Freezing Media At Morula Stage Towards The Percentage Of Embryonic Development At Blastula Stage
  3. The Liver Histopathology Of Mice (mus Musculus) Cause Exposure Carbofuran Insectiside
  4. Effect Of Mangosteen Rind Extract (garcinia Mangostana L.) To Necrosis Alveolar Type Ii Cell Of Lungonpregnant Mice (mus Musculus)exposured By Cigarette Smoke
  5. Histopathological Appearance Of Gastrointestinal Tract Iv Instar Mosquito Larvae Of Aedes Aegypti After Dipping With Active Compounds Of Permot Leaf Extract (passiflora Foetida Linn.) And Its Potential As Bioinsecticide
  6. The Effects Of Differences In The Duration Of The Thawing Of Frozen Semen To Sperm Quality Peranakan Ettawa Goat
  7. The Seminal Plasma Protein Of Cow Sustain Quality Of Beans Goat Spermatozoa After Equilibration In Freezing Process
  8. The Potential Of Garlic (allium Sativum) Infuse To Caecum Of Broilers Histopathology Infected By Escherichia Coli
  9. The Effectiveness Of Giving Haemopoetic Stem Cells In The Pregnant Mice (mus Musculus) Exposed To Plumbum Towards The Decreasing Number Of Necrocis Cells And Expression Of Caspase-3 In Placenta
  10. Protective Effect Of Binahong Leaf Extract [anredera Cordifolia (ten.) Steenis] To Renal Histopathological Of Rat (rattus Norvegicus) Induced With Borax (na2b4o710h2o)
  11. Effect Of Antibacterial Traditional Apple Vinegar And Apple Vinegar Products On The Growth Of Staphylococcus Aureus In Vitro
  12. The Effect Of Mangosteen Peel Extract (garcinia Mangostana L.) To The Number Of Placental Trophoblast Cells In Mice (mus Musculus) Exposure To Cigarette Smoke
  13. Analysis Of Humoral Immune Response (igg) In Rabbit Immunized With Proteins Of Leucocytozoon Caulleryi
  14. Isolation Of Methicillin-resistant Staphylococcus Aureus (mrsa) In Dog Nasal Swab With Oxacillin Screen Agar Confirmation Test
  15. Effect Of Prostaglandin F2α, Pregnant Mare Serum Gonadotropin, And A Combination Of Pregnant Mare Serum Gonadotropin And Human Chorionic Gonadotropin On Pregnancy Rates And Litter Size Of Mice
  16. Through The Provision Of Drinking Water Multiprobio Against Performan Broiler Chicken Production

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