Veterinaria Medika
ISSN 1979-1305
Vol. 2 / No. 1 / Published : 2009-02
Order : 4, and page :17 - 20
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Original Article :
Optical density value of antibody against pregnancy-associated glycoproteins (pags) from the pregnant dairy cow blood serum in male local rabbit (oryctolagus cuniculus)
Author :
- Tita Damayanti Lestari*1
- Setyarina Indrasari*2
- Pudji Srianto*3
- Ajik Azmijah*4
- Departemen Ilmu Peternakan Universitas Padjajaran Bandung
- PPDH Fakultas Kedokteran Hewan Universitas Airlangga
- Fakultas Kedokteran Hewan Universitas Airlangga
- Fakultas Kedokteran Hewan Universitas Airlangga
Abstract :
The aim of this study was to examine if PAGs could induce anti-PAGs antibody based on theoptical density (OD) value measured with Indirect ELISA. Five male rabbits were subcutaneously immunized with 100 µg PAGs in CFA at week-0 and boostered 100 µg PAGs in IFA at week-3 and week-8. Blood samples were collected from Auricular vein subjected to anti-PAGs analyze by means of indirect ELISA. Result obtained were analyzed as descriptive data. This study showed that immunization utilized PAGs isolate could induce anti-PAGs antibody. The highest OD value was obtained at week-11 after immunization with OD value 1.044.
Keyword :
OD value, PAGs, Anti-PAGs, male rabbits, .,
References :
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