UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 4 / No. 1 / Published : 2011-02

Order : 6, and page :25 - 36

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Original Article :

Pengaruh pemberian antagonis reseptor n-metil-d-aspartat (nmda) mk-801 terhadap penurunan sensasi nyeri inflamasi pada mencit putih (mus musculus) strain balb/c

Author :

  1. Susilo, Imam *1
  2. Gondo, Lisa *2
  3. Aji, Rahmad *3
  4. Bambang S.Z*4
  5. Khotib, Junaidi*5
  1. Departemen Patologi Anatomi, Fakultas Kedokteran Universitas Airlangga
  2. Departemen Farmasi Klinik, Fakultas Farmasi Universitas Airlangga
  3. Departemen Farmasi Klinik, Fakultas Farmasi Universitas Airlangga
  4. Departemen Farmasi Klinik, Fakultas Farmasi Universitas Airlangga
  5. Departemen Farmasi Klinik, Fakultas Farmasi Universitas Airlangga

Abstract :

Chronic pain, such as inflammatory pain, is difficult to manage. N-Methyl-D-Aspartate (NMDA) receptor is a key molecule involved in the pain pathway and sensitization. The present study was design to investigate the efficacyof a novel potent non-competitive NMDA receptor antagonist, MK-801, in relieving inflammatory pain in mice. A model of inflammatory pain state was induced by intraplantar injection of Complete Freund's Adjuvant (CFA). NMDA receptor antagonist MK-801 was administered intrathecally once a day for 7 consecutive days at 0.01, 0.10, 1.00, 10.00, or 20.00 nmol doses a week after CFA injection. Thermal hyperalgesia was measured on days 0, 1, 3, 5, 7,8, 10, 12, 14, and 21 after CFA injection by warm plate. Paw thickness at the ipsilateral site was also measured on days 0, 1, 2, 3, 4, 5, 6, 7, 8, 10, 12, 14, and 21 after CFA injection. Histology of the spinal cord tissue was examined by lightmicroscope following haematoxylline-eosin staining. The results showed that MK-801 given at 0.01-20.00 nmoldosage significantly increased mice's latency on thermal stimulation compared with placebo (p<0.001). Pawthickness was also significantly decreased to compare with placebo after intrathecal injection of MK-801 at 0.01, 1.00and 10.00 nmol dosage (p<0,001; p=0,005; p=0,015 respectively). Whereas MK-801 administration at 0.01, 1.00, and20.00 nmol could decrease the inflamatory cells infiltration and recover the dorsal horn histology compare with placebo. Taken together, these results show that NMDA receptor antagonist, MK-801, is effective in relieving the inflammatory pain.

Keyword :

Chronic Pain, Inflammatory Pain, MK-801, NMDA receptor, .,


References :

Lipton, S., A.,(2004) Failures and Successes of NMDA Receptor Antagonists: Molecular Basis for the Use of Open- Channel Blockers like Memantine in the Treatment of Acute and Chronic Neurologic Insults United States : J. Am. Soc. Exp. Neuro Therapeutics, 1 :101-110.

Kocaeli, H., E. Korfali, H. Öztürk, N. Kahveci dan S. Yılmazlar,(2005) MK-801 Improves Neurological and Histological Outcomes after Spinal Cord Ischemia Induced by Transient Aortic Cross-Clipping in Rats United States : Surgical Neurology, 64 (2) : S22-S26.

Alilain, W. J. dan H. G. Goshgarian,(2007) MK-801 Upregulates NR2A Protein Levels and Induces Functional Recovery of the Ipsilateral Hemidiaphragm Following Acute C2 Hemisection in Adult Rats United States : The Journal of Spinal Cord Medicine. 30 (4) : 346-354





Archive Article

Cover Media Content

Volume : 4 / No. : 1 / Pub. : 2011-02
  1. Production And Specificity Testing Of Monoclonal Antibodies To Bovine Zonna Pellucida 3 Deglycosylated (mab-bzp3dg) For Woman Immunocontraceptive Vaccine
  2. Potency Of Crude Spirulina On Protein Efficiency Ratio In Laying Hen
  3. H-y Antisera Preparation And X Chromosomal Receptor Tracer As Sex Determination Prototype
  4. Erythrocyte's Form Changes In Dog's Blood Smear Before And After The Storage Using Citrate Phosphate Dextrose
  5. Avian Influenza H5n1 Vaccine Candidate For Chicken From East Java Isolate Virus
  6. Pengaruh Pemberian Antagonis Reseptor N-metil-d-aspartat (nmda) Mk-801 Terhadap Penurunan Sensasi Nyeri Inflamasi Pada Mencit Putih (mus Musculus) Strain Balb/c
  7. Exploration Cellulolytic Of Bacterium Of Rumen Liquid Beef Cattle As Inoculum Of Waste Agriculture
  8. Respon Hmg Toward Ovarium Development In Goat
  9. Identification Of Pregnancy Associated Glycoprotein (pag) From Milk Of Pregnant Dairy Cattle
  10. The Role Of Soil As A Helminths Transmitter Around The Habitas Of Babirusa
  11. Potency Of Brown Seaweed (sargassum Duplicatum Bory) Ethanol And Ethyl Acetic Fraction To Malondialdehyde Concentration Decreasing And Histological Retriveal Of Ibd (inflammatory Bowel Disease) Rat Small Intestinal Jejunum
  12. Blood Glucose And Total Blood Protein Profile In Sheep Provided With Lactic Acid Bacteria And Yeast On King Grass And Rice Straw
  13. Effect Of Pegagan (centella Asiatica) Extract In Ovariectomized Wistar-strain Rattus Norvegicus On Epithelial Proliferation Of Vaginal Wall
  14. Excessive Dose Of Vitamine A On Skeletal Development In The Mice Embryos

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