Veterinaria Medika
ISSN 1979-1305
Vol. 7 / No. 3 / Published : 2014-11
Order : 6, and page :240 - 247
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Original Article :
Potency of pediococcus pentosaceus protease on meat tenderness and histological
Author :
- Kun Muliati*1
- Nenny Harijani*2
- Thomas Valentinus W.*3
- Mahasiswa Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
Abstract :
Tenderness is a determining factor of customer satisfaction. Protease enzyme used for meat tenderizer through protein degradation. Pediococcus pentosaceus is a lactic acid bacteria that have been known to produce extracellular protease enzymes. This study aims to determine potency of Pediococcus pentosaceus Protease On Meat Tenderness And Histological. Meat samples were divided into three groups, one group is immersed in a solution of a commercial papain enzyme, another group immersed in a protease enzyme from Pediococcus pentosaceus and last group as a control. After incubation for 60 min, the tenderness of the meat was measured using a penetrometer. Statistical test showed there were significant differences p< 0.05 between the control with treated meat. Treatment with papain enzyme showed the highest level of tenderness, although not significantly different from the sample is added enzyme protease from Pediococcus pentosaceus. Histological examination showed degeneration of muscle fibers in both treatment groups compared to the control. bonds between the muscle fibers is lose, the muscle fibers easily disconnected and separated so that the meat becomes tender.
Keyword :
Protease enzyme, Pediococcus pentosaceus, Meat tenderizer, Meat histological,
References :
Ionescu, A., I. Apodu and G. Pascoru,(2008) Effect of Papain and Bromelin on muscle an Collagen protein in Beef Meat . : Food Technology. 31 : 9-16
Suri, W.L., S. Syukur dan Jamsari,(2013) Optimization of Protease Activity Lactidacid Bacteria (LAB) Pediococcus Pentosaceus Isolated from Soursop Fermentation (Annona Musicata L.) Padang : Jurnal Kimia Unand
Ikram-Ul-Haq and H. Mukhtar,(2013) Protease Biosynthesis from Lactobacillus Species: Fermen-tation Parameters And Kinetics Pakistan : Institute of Industrial Biotechno-logy government College University Lahore
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