Veterinaria Medika
ISSN 1979-1305
Vol. 7 / No. 1 / Published : 2014-02
Order : 3, and page :17 - 22
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Original Article :
Potential of phytase ezymes from rumen bacteria on sem analysis structural changes of rice bran as broiler feed
Author :
- Mirni Lamid*1
- Ni Nyoman Tri Puspaningsih*2
- One Asmarani*3
- Dosen Fakultas Kedokteran Hewan
- Dosen Fakultas Sains dan Teknologi
- Instiute Tropical of Diseases Universitas Airlangga
Abstract :
Phytase enzymes is one belonging to a group that is able to hydrolyze phosphatase in the form of phytate compounds Myo - inositol (1,2,3,4,5,6) hexsa into myo - inositol phosphatase and organic phosphate . In the gastrointestinal tract of non -ruminant livestock (poultry) there is no phytase enzyme , it causes the content in rice bran phytate compounds are difficult to digest because of the strong chelating properties , so the phytate wasted with feces (stool) . Restrictions on the use of rice bran in the diet because the fiber content and high phytic acid . One alternative to reduce the phytate content of the feed is to use phytase enzyme producing bacteria. Rumen bacteria (Actinobacillus sp and Bacillus pumilus) were expected to produce the enzyme phytase to hydrolyze phytic acid (myo-inositol 1,2,3,4,5,6-hexakisphosphate) rice bran to produce a series of orthophosphate in organic and phosphoric more low (inositol pentaphosphate to monophosphate) and myo - inositol eventually become free, so that all minerals such as P, Ca, Mg, Fe, and Zn were an essential mineral to be released and used for the growth of broiler chickens. The result of research can be concluded that Scanning Elektron MicroscopeScanning Elektron Microscope (SEM) analysis of the surface with the addition of rice bran phytase enzyme causes bond breaking force with myo-inositol phosphate acid groups that show changes in the structure of phytic irregular bond
Keyword :
Actinobacillus sp, Bacillus pumilus, Enzyme phytase, Rice bran, Phytic acid,
References :
Graft, E.,(1983) Phytic acid a natural antioxidant USA : J. Biol. Chem. 262(24) : 11647-11650
Kurniati, A,(2011) Analisis SEM dan XRD terhadap perubahan struktur permukaan dan kristalinitas jerami padi dan enceng gondok akibat aktivitas ά-L-Arabinofuranosidase rekombinan Surabaya : Tesis. Fakultas Sains dan Teknologi. Universitas Airlangga
Mirni L, N.N. Tri Puspaningsih dan Widya Paramita L,(2006) Penggunaan Bakteri Xilanolitik Asal Rumen Sebagai Inokulum pada Jerami Padi sebagai Upaya peningkatan Mutu Pakan Ternak Ruminansia Surabaya : Lembaga Penelitan Universitas Airlangga.
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