UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 1 / No. 2 / Published : 2008-06

Order : 3, and page :12 - 18

Related with : Scholar   Yahoo!   Bing

Original Article :

Protein characterization of insulin like growth factor – i complex of goat seminal plasma as an capacitation medium of sperm

Author :

  1. Suherni Susilowati*1
  1. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

Nineteen percentage of goat semen consist of seminal plasma. Seminal plasma reported to be important for maintaining sperm motility, sperm viability and for increasing the resistance to cold damage. Seminal plasma, a physiological secretion from multiple glands of the male reproductive tract, is the natural medium for maturation, enzymatic and surface-modifiing events. Seminal plasma contains a variety of biochemical components some of which are relatively specific for the regulation of spermatozoa function. The addition and removal of variety of protein during epididymal maturation and at ejaculation play important roles in the capacitation of sperm and fertilizationof the egg. In this article it will be discussed about the protein characterization of goat seminal plasma by Sodium Dodesil Sulfat – Poliakrilamid Gel (SDS-PAGE) 12% procedure. Fractination of goat seminal plasma protein by SDS-PAGE procedur would be result seven fractions. First fraction is with molecul mass 150,288 kDa, second fraction is with molecul mass 103,486 kDa, 3rd fraction is with molecul mass 76,155 kDa, 4th fraction is with molecul mass 53,249 kDa, 5th fraction is with molecul mass 35,378 kDa, 6th fraction is with molecul mass 14,099 kDa and 7th fraction is with molecul mass 11,492 kDa. The further demonstrate the first fraction with molecul mass 150 kDa is ternary protein complex consist of a molecul IGFBP-3 (53 kDa), a molecul IGF-I or IGF-II (7,5-10 kDa) and unique acid labil subuinit/ALS (76 kDa). In conclution of this study is goat seminal plasma protein component consist of seven fractions and that it’s could be to maintain the sperm.

Keyword :

Goat, Seminal plasma protein, IGF-I, SDS-PAGE, .,


References :

Aulani’am,(2005) Protein dan Analisisnya Malang : Citra Mentari Group

Dorothy, E.S,(1993) Intisari Biokimia Jakarta : Binarupa Aksara





Archive Article

Cover Media Content

Volume : 1 / No. : 2 / Pub. : 2008-06
  1. Prevalence Rate Of Bovine Mastitis From Several Dairy Herds In East Java
  2. The Sambiloto Leaf As An Active Subtance Of Dipping Solution On A Dairy Cattle Mastitis
  3. Protein Characterization Of Insulin Like Growth Factor – I Complex Of Goat Seminal Plasma As An Capacitation Medium Of Sperm
  4. The Influence Of Infected Oocyst Of Eimeria Tenella On Meat Quality Of Broilers
  5. Increasing Yoghurt Flavour Dan Texture From Goats Milk By Adding Inoculan Consentration
  6. The Sensitivity Test Of Several Antimicrobial Agents To Escherichia Coli That Caused Mastitis In Dairy Cows At Around Of Suka Makmur Dairy Cooperative Grati Pasuruan
  7. Sex Determination From Mandible By Angular Morphometric Methods On Goat (capra Spp.)
  8. Identification Of Protein Growth Differentiation Factor-9 (gdf-9) Isolated From Oocytes Dominant Follicles Of Bovine Ovum
  9. Effect Of Teratogenic Insecticide Carbofuran At Chicken Embryo
  10. The Addition Of Heparin And Hypotaurine In Capacitation Medium Earle’s Balanced Salt Solution (ebss) On Embryo Development In Vitro Fertilization Of Dairy Cattle
  11. The Influence Of 60% Ethanol Fraction And Water Fraction Of Justicia Gendarussa Burm.f Leaf On Pegion (columba Livia) Emetic Activity
  12. Biosynthesis And Regulation Of Sex Pheromones (z)-9-tricosene In The Housefly, Musca Domestica
  13. Detection Of Rabbits Serum Antibody Which Were Immunized By Sarcoptes Scabiei Var. Caprae

Information index.php/JIPK/VETMED