UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 7 / No. 2 / Published : 2014-07

Order : 6, and page :134 - 139

Related with : Scholar   Yahoo!   Bing

Original Article :

Spesifisity test with dot blotting of epidermal growth factor (egf) isolated from cumulus oocyte complex after in vitro maturation

Author :

  1. Widjiati*1
  2. Aulia Reza Pradipta*2
  3. Dady Soegianto Nazar*3
  4. A.T. Soelih Estoepangestie*4
  1. Dosen Fakultas Kedokteran Hewan
  2. Mahasiswa Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan
  4. Dosen Fakultas Kedokteran Hewan

Abstract :

The aim of this study was to know the character of epidermal growth factor (EGF) protein isolated from bovine oocyte. Bovine ovary was collected from slaughter house washed with NaCl. An aspiration technique was used to collect oocyte. Those oocytes were maturated by in vitro condition then the EGF protein was isolated after running in SDS PAGE. Polyclonal antibody was produced by sub cutaneous injected of crude protein oocytes containing EGF in rabbit, then followed by dot blotting examination. Booster was done twice after 3rd and 7th bleeding then continued until 10 times every week. The antibody anti EGF from rabbits sera was reacted with EGF antigen. The dot blot showed, the intensity of dot color increased significantly from 4th bleeding and reaches the highest concentration of antibody at 9th bleeding. It meant, bovine oocyte EGF protein could be characterized by dot blotting, of which the highest antibody titer detected at eleventh week after sub cutaneous injection of crude protein oocytes containing EGF.

Keyword :

Epidermal Growth Factor, In vitro Maturation, Dot Blotting,


References :

Kobayashi, K., S.Yamashita and H. Hoshi,(1994) Influence of Epidermal Growth Factor And Transforming Growth Factor on In Vitro Maturation of Cumulus Cell-Enclosed Bovine Oocytes in a Defined Medium . : J. Reprod. Fertil. 100: 439-446

Lorenzo, P.L., I.K.M. Liu, J.C.Illera, R.A.Picazo, G.F.Carneiro, M.J. Illera, A.C. Conley, A.J.Ender,(2001) Influence of Epidermal Growth Factor on Mammalian Oocyte Maturation via Tyrosine-Kinase Pathway . : J. Physiol. Biochem., 57 (1): 15-22

Baratawidjaja, K.G,(2006) Imunologi Dasar Jakarta : Penerbit Fakultas Kedokteran Universitas Indonesia





Archive Article

Cover Media Content

Volume : 7 / No. : 2 / Pub. : 2014-07
  1. The Influence Of Carbofuran Exposure Toward White Pulp Diameter Of Spleen Of Mice (mus Musculus)
  2. Antibacterial Activity Of The Supernatant Of Soil Isolate Bacillus Subtilis Against Aeromonas Hydrophila And Staphylococcus Aureus (in Vitro)
  3. The Potential Of Giving Synbiotic In Different Ages Of Female Broilers On Histological Of Ileum
  4. Evaluation Toluidine Blue Staining To Identify Connective Tissue Mast Cells (ctms) In Paraffin Block Thin Skin Of Dog
  5. Effect Using A Combination Of Mpa (medroxy Progesterone Acetate) And Prostaglandin (pgf2α) Injection On The Percentage Of Estrous And Pregnant On Sheeps
  6. Spesifisity Test With Dot Blotting Of Epidermal Growth Factor (egf) Isolated From Cumulus Oocyte Complex After In Vitro Maturation
  7. Analysis Of Immunogenicity On Inactivated Dengue Virus (denv-1, Denv-2, Denv-3, Denv-4) In Mices (mus Musculus) As A Candidate Dengue Coctail Vaccine
  8. Detection Antibody Of Brucella On Cattle Slaughtered In Krian Slaughter House Sidoarjo Regency By Rose Bengal Test (rbt)
  9. The Effect Of Didecyldimethylammonium Chloride Disinfectants On Liver Histophatolologycal Of Duck Hybrid (anas Platyrhynchos Domesticus)
  10. The Effect Of Cosmos Caudatus Leaf Ethanol Extract On Paracetamol Induced In Histopathologic Liver Of (mus Musculus) Balb / C Male
  11. Antibacterial Test Of Rumbia Root (metroxylon Sagu Rottb.) Decoction Against Bacteria Salmonella Pullorum
  12. Effect Of Sambiloto Leaf Extract (andrographis Paniculata Ness) To Histopathological Pancreatic Langerhans Islet Cells On Rats (rattus Norvegicus) With Cystic Ovary Model
  13. Effect Of Mangosteen (garcinia Mangostana L.) Pericarp Extract On Tlr5 And Cd14 Expression In Immunized Mice Against Newcastle Disease Vaccine
  14. Cloning Gene Fragments Non-structural 1 (ns1) Of Dengue Virus Subtype 1 (denv-1) As A Material Candidate Of Vaccine Chimera
  15. Effect Of Vitamin E (α–tocopherol) On The Number Of Leydig Cell In Mice Treated With 2,3,7,8–tetrachlorodibenzo-p-dioxin (tcdd)

Information index.php/JIPK/VETMED