UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 3 / No. 2 / Published : 2010-07

Order : 9, and page :123 - 126

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Original Article :

Supplementation of insulin like growth factor – i complex protein of goat seminal plasma to quality of acrosome cap of sperm after centrifugation

Author :

  1. Suherni Susilowati*1
  1. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The aim of this research was to study the supplementation Protein Insulin Like Growth Factor –I Complex seminal plasma on quality of acrosome sperm after centrifugation. First experiment was conducted to isolate Protein Insulin Like Growth Factor – I Complex from goat seminal plasma . Second experiment was conducted to role of Protein Insulin Like Growth Factor – I Complex to quality of acrosome cap of sperm after centrifugation. Semen was collected and then added with Bracket and Oliphant (BO) medium and centrifugated for 5 minutes 600 G. And then pellet of sperm divided into three groups. The first, second and third group supplemented with 3 x 106 of spermconcentration. All of groups incubated for 30 minutes and 45 minute in BO medium, BO medium plus Protein IGF-I Complex and Protein IGF-I Complex medium respectively. This sperm was characterized based to quality of acrosome cap of sperm. The result showed that the percentage to quality of acrosom cap of sperm after centrifugation was significantly different in three groups (p<0.05), which the highest to quality of acrosome cap of sperm was found in Protein IGF-I Complex medium. It was concluded that Protein IGF-I Complex of goat seminal plasma could increase toquality of acrosome cap of sperm.

Keyword :

Acrosome cap of sperm, seminal plasma , IGF-I Complex, ., .,


References :

Aulani,am,(2005) Protein dan Analisisnya Malang : Citra Mentari Group

Alvarez JG and BT Storey,(1995) Differential Incorporation of fatty Acid Into and Peroxidative Loss of Fatty Acid from Phospholipid of Human Spermatozoa United States : Mol.Reprod.

Anastasia D, Mistry J, Krishna RG dan Khosravi J,(2000) Immunoassay of Insulin Like Growth Factor Binding Protein 3 (IGFBP-3) New Means to Quantifiying IGFBP3 Proteolysis United States : J Endocrin and Metabolis





Archive Article

Cover Media Content

Volume : 3 / No. : 2 / Pub. : 2010-07
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  2. Detection Of Inflamentation On Duck Moulting Therapy By Anti-prolactine Injection
  3. The Effects Of Eucheuma Spinosum Extracts On The Sod (superoxide Dismutase) Activities On Mice (rattus Novergicus) Induced Multiple-low Dose Streptozotocin
  4. Ethanol Extract Of Brown Secured (sargassum Duplicatum Bory): Phytopharmaco Exploration For Lung Cancer Therapy
  5. Using Logistic And Gompertz Model To Estimate The Body Weight Growth Of Lohmann Broiler Chicken
  6. Case Study : Suspect Feline Infectious Peritonitis (fip) Of Cat In Surabaya
  7. The Usefull Of Rice Straw, Ammoniation Rice Straw And Soybean Straw As Single Fed On Microbial Protein Synthesis Of Ongole Grade Cattle
  8. Kajian Lintas Seksional Selang Beranak (calving Interval) Pada Tingkat Ternak Dan Peternak Pada Sapi Perah Di Jawa Timur
  9. Supplementation Of Insulin Like Growth Factor – I Complex Protein Of Goat Seminal Plasma To Quality Of Acrosome Cap Of Sperm After Centrifugation
  10. Concentration Of Pregnancy Associated Substances (pas) Of Ettawa Crossbreed Goats On Several Stage Of Pregnancy
  11. Motility And Viability Spermatozoa Muscovy Duck (cairina Moschata) On The Various Concentration Of Egg Yolk Fructose And Skim Milk Diluter
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  13. Isolation And Identification Of Coagulase-positive Staphylococcus From Injury On Dog Skin In Surabaya
  14. Influence Of Melatonin To Histopathology Pancreas In Rat ( Rattus Norvegicus ) Experimentally Diabetes Mellitus Type I

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