Veterinaria Medika
ISSN 1979-1305
Vol. 5 / No. 1 / Published : 2012-02
Order : 5, and page :17 - 20
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Original Article :
The activity test of whole bacterium suspension of bacillus thuringiensis subspecies israelensis serotype h-14 at mosquito of culex fatigans pipiens and aedes aegypti
Author :
- Hamid, I.S*1
- Fakultas Kedokteran Hewan Universitas Airlangga
Abstract :
The aim of this research is to know the amount of death of adult mosquito at two different species that is Culex pipiens fatigans and Aedes aegypti which is sprayed by whole bacterium of Bacillus thuringiensis subspecies israelensis serotype H-14. Attempt animal at this research is 200 adult mosquito to each; every species devided into4 group with same amount and each; every group packed into fairish glass box 25x25 cm. Each group are sprayed by 2 ml suspension of whole bacterium 107,108,109 concentration which is added by aquades till 20 ml. Control group sprayed by aquades 20ml. The death rate of mosquito counted 24 hours after treatment. This research useCompletely Randomized Design with 3 repetitions. Data result of research in the form of amount of death of mosquito which analysed by Analysis of Variance (Anova). And continued with Honestly Significant Difference (HSD) if result is different. Result of research indicate that there are no difference death amount of mosquito which is sprayed by whole bacterium with different concentration 107,108, 109 and control of Culex pipiens fatigans and also Aedes aegypti.
Keyword :
Bacillus thuringiensis subspecies israelensis serotype H-14, Culex pipiens fatigans, Aedes aegypti, Whole bactery,
References :
Croft, Brian A.,(1990) Arthropod Biological Control Agents and Pesticides Canada : Willey Interscience Publication John Willey & Sons Inc.
Hamid, Iwan Sahrial., Susilohadi W., Mufasirin,(2004) Profil, Produksi dan Aktifitas Protein_Endotoksin dari Bacillus thuringiensis subspecies israelensis serotype H-14 sebagai insektisidal pada Berbagai Larva Nyamuk Surabaya : Laporan Penelitian Hibah DUELIKE Batch III
Pietrantonio, V. Patricia, A. Brian Federici, Sarjeet and S. Gill,(1993) Interaction of Bacillus thuringiensis Endotoxin with The Insect Midgut Epithelium. Parasites and Pathogens of Insects. California. : Academic Press Inc. 55-74pps
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