UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 7 / No. 1 / Published : 2014-02

Order : 4, and page :23 - 30

Related with : Scholar   Yahoo!   Bing

Original Article :

The comparison of morphology between bovine oocyte vitrification before and after in vitro maturation

Author :

  1. Zakiyatul Faizah*1
  2. Aucky Hinting*2
  3. Ninik Darsini*3
  4. Widjiati*4
  1. Mahasiswa Fakultas Kedokteran
  2. Mahasiswa Fakultas Kedokteran
  3. Mahasiswa Fakultas Kedokteran
  4. Dosen Fakultas Kedokteran Hewan

Abstract :

Vitrification method has become a growing and promising technology in assisted reproductive technology. Freezing embryos are more common, freezing oocytes are longer growing because the physical characteristics of oocyte. The spindle of oocytes can be maintained during the process of freezing and warming, although exposed to very cold temperatures and undergo twice transition phase.This study aims to compare the number of morphology between bovine oocyte vitrification pre and post in vitro maturation. Maturation is operated in medium TC 100 μl covered with mineral oil in a petri dish with diameters 36 mm. Vitrification begins with washing oocyte in PBS of 20% serum for 1-2 minutes, followed by PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred in 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing is processed by submerging the oocytes in the media: 1). PBS + 20% serum + 0.5 M sucrose, 2). PBS + 20% serum + 0.25 M sucrose, and 3). PBS + 20% serum + 0.1 M sucrose.This study conclude that no difference on normal and abnormal oocyte morphology between bovine oocyte vitrification pre and post in vitro maturation.

Keyword :

Vitrification, In vitro maturation, Morphology,


References :

Alworth AE and D.F. Albertini,(1993) Meiotic maturation in cultured bovine oocytes is accompanied by remodeling of the cumulus cells cytoskeleton . : Development Biology. 158:101-112

Bogliolo L, F. Ariu, S. Fois , I. Rosati , M.T. Zedda , G. Leoni , S. Succu , S. Pau and S. Ledda,(2007) Morphological and Biochemical Analysis of Immature Ovineoocytes Vitrified With or Without Cumulus Cells. Theriogenology. USA : Theriogenology. 68: 1138-1149

Gardner, DK, B.R.M.B Rizk and T. Falcone,(2011) Human Assisted Reproductive Technology: Future Trends In Laboratory and Clinical Practice UK : Cambridge University Press. 314





Archive Article

Cover Media Content

Volume : 7 / No. : 1 / Pub. : 2014-02
  1. Grant Of Ethanol Extract Of Seeds Papaya (carica Papaya) In Female Rats (rattus Novergicus) Antifertilitas Material, As An Alternative To Number And Quality Of Egg Cells
  2. Effect Of Infusum Salam Leaves (eugenia Polyantha) On Blood Glucose Level On The Rat (rattus Norwegicus) In Alloxan Induced
  3. Potential Of Phytase Ezymes From Rumen Bacteria On Sem Analysis Structural Changes Of Rice Bran As Broiler Feed
  4. The Comparison Of Morphology Between Bovine Oocyte Vitrification Before And After In Vitro Maturation
  5. The Effect Of Exposing Pregnant Rat (rattus Novergicus) To Particulate Matter On Congenital Disorder And Resorption Of Embryos
  6. Ha Gene Phyloanalysis Of Influenza H1n1 2009 Virus In Surabaya
  7. Effect Of Seminal Plasm Of Cow On Goat’s Spermatozoa Plasma Membran In Milk Diluter On Semen Freezhing
  8. Histopathological View Pyramid Cell On Cerebrum Of White Rat (rattus Norvegicus) Were Exposed With Methylmercury Which Administrated With Cdp-choline As Neuroprotective Agent
  9. Giving Epigallocathechin Gallate (egcg) Toward The Expression Of Estrogen Receptor At Female White Mouse (rattus Norvegicus)
  10. Human Resource Management Goad Farm Of The Cross Breed Etawa Goat Independent Farm, Farmer Groups And Businesses In The Town Of Turi Sleman Regency
  11. The Pattern Of Antimicrobial Susceptibility Of Salmonella Sp Isolated From Beef
  12. Potential Mycotoxin Binders On Histopathology Of The Mammary Gland Experiencing Neoplasia In Pregnant Mice (mus Musculus) With Exposed Zearalenone
  13. Potential Of Mycotoxin Binders On Weight And Length Of Fetus Pregnant Mice (mus Musculus) With Zearalenone Exposure
  14. Prevalence Of Worm Disease In Cattle Gastrointestinal Peranakan Ongole (po) And Simental Cattle In The Subdistrict Laren, District Lamongan
  15. The Giving Of Anti- Toxoplasma Gondii Membran Immunoglobulin Y Before Infection Reduce Apoptotic Index Liver Mice

Information index.php/JIPK/VETMED