UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 1 / No. 1 / Published : 2008-02

Order : 5, and page :23 - 28

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Original Article :

The effect of alkaloid fraction of jarong (achyrantes aspera linn) on viability culture myeloma cell mice

Author :

  1. Dewa Ketut Meles*1
  2. Dhevie Kenny Astarina*2
  3. Suwarno*3
  4. Husni Anwar*4
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga
  4. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The purpose of this research is to prove that the effect of alkaloid fraction of jarong (Achyrantes aspera linn)can be used as a cure for cancer with invitro on cultured cell of myeloma cell of mice using the method of cellcounting. In this research, the percentage of the perceived cell viability of the living myeloma cell is comparedwith the dead cell using the negative and positive control. This research divided into five group: P0 is controlnegative,P1 liquid test 1 ppm, P2 liquid test 10 ppm, P3 liqiud test 100 ppm, P4 liquid test 1000 ppm and P5positive control of 100 ppm colchisin. All groups were repeated four times and incubated for 24 hours in anincubator 95% O2 5% CO2 at temperature of 37 OC. Then the culture which had been treated was taken 0,1ccand give 0,1cc of 4% tripan blue. Later the dead cells and the living cell were calculated using the thomamethod with the help of hemositometer. The living cells will be colorless and dead cell will be tinted.The resultof assasment of the myeloma cells using hemositometer showed that the cultured cell in the concentration of100 ppm and 1000 ppm will have lower viability compared with the concentration of 1 ppm, 10 ppm and positivecontrol. In the concentration 1 ppm, the alkaloid fraction of jarong (Achyrantes aspera linn) killed more than 50percentage of myeloma. The alkaloid fraction of jarong (Achyrantes aspera linn) on the viability of myeloma cellculture of mice will have Lethal Concentration 50 : 0,716 ppm.

Keyword :

viability cell, cultured cell, myeloma, Achyrantes aspera linn, .,


References :

Adyana, I.D.P.,(2006) Efek Anti Telomerase Fraksi Alkaloid Terhadap Pembelahan dan Mitosis Sel Mieloma Mencit Surabaya : Fakultas Kedokteran Hewan Universitas Airlangga

Freshney, L.R.,(1987) Culture of Animal Cell : A Manual Basic of Technique New York : Alan R. Liss Inc.





Archive Article

Cover Media Content

Volume : 1 / No. : 1 / Pub. : 2008-02
  1. Identification Of Staphylococcus Aureus Which Causes Mastitis With Mannitol Test And Detection Of Acetoin Product In Dairy Cattke At The Farm Area Of Suka Makmur Dairy Cooperative Grati Pasuruan
  2. The Influence Of Api - Api (avicennia Marina) Leaves Extract Toward Embryo Resorbtion, Body Weight And Lengh Toetal Mice (mus Musculus)
  3. The Effects Of Eimeria Tenella Infection On The Spleen Weight, Size And White Pulp Diameters In The Chickens Primary Infection And Secondary Infection
  4. Detection Of Avian Influenza Virus Subtype H5 On Stray Cats (felis Silvestris Catus) In Bandung City Area
  5. The Effect Of Alkaloid Fraction Of Jarong (achyrantes Aspera Linn) On Viability Culture Myeloma Cell Mice
  6. Antibacterial Activity Of The Ethanol Extract Pluchea Indica Less Leaves Against Escherichia Coli By In Vitro
  7. Identification Of Protein Profile Of Sarcoptes Scabiei On Goats By Sds-page Analysis
  8. Identification Of Excretion-secretion Protein Profile Of The Adult Haemonchus Contortus With Sds-page
  9. Identification Of Aerob Cellulolytic Fungi From Rumen Fluid Waste At Pegirian Slaughter House Surabaya
  10. The Effects Of Supplying Commercial Probiotic On The Quantity And Quality Of Dairy Cow Milk
  11. The Effect Of Given Crude Chlorella To Total Cholesterol Blood Rates On Broiler Chicken
  12. The Effect Sgot And Sgpt Level Of Whiterat ( Rattus Norvegicus ) That Was Given Juice Of Noni ( Morinda Citrifolia ) And High Fatty Diet
  13. Utility Information Technology ( It ) For Head Surface Area Of Spermatozoa Sheep With Biometric Measurement Methode
  14. Influence Of Leaf Extract Of Sirih ( Piper Betle L.) To Growth Staphylococcus Aureus And Escherichia Coli With Disk Diffusion Method
  15. Protein Characterization Of Excretory Secretory Second Stage Larvae Dorman Against Antibody Anti-second Stage Larva Toxocara Canis By Western Blot Techniques

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