Veterinaria Medika
ISSN 1979-1305
Vol. 4 / No. 2 / Published : 2011-07
Order : 1, and page :87 - 90
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Original Article :
The effect of tyrosine kinases addition into the egg yolk skim and egg yolk tris extender the motility and live spermatozoa percentages of the holstein friesian post thawing
Author :
- Madyawati, S.P.*1
- Srianto, Pudji*2
- Dwi, C.P*3
- Ismudiono,*4
- Sianita, Nanik*5
- Fakultas Kedokteran Hewan Universitas Airlangga
- Fakultas Kedokteran Hewan Universitas Airlangga
- PPDH Fakultas Kedokteran Hewan Universitas Airlangga
- Fakultas Kedokteran Hewan Universitas Airlangga
- Fakultas Kedokteran Hewan Universitas Airlangga
Abstract :
The aim of this study is to determine the effects of the tyrosine kinase addition into the egg yolk skim and egg yolk tris extenders on the motility and live percentages of the thawed Holstein Friesian (FH) semen. This study used fresh Holstein Frisian semen collected using artificial vagina then this semen divided into four treatment groups. For the first group (P1) semen was diluted in egg yolk skim contained 100 ug / ml tyrosinekinase. For the second group (P2) semen was diluted in egg yolk skim extender contained1,2% NaCl. For the third group (P3) semen was diluted in egg yolk tris extender contained 100 ug/ml tyrosine kinase. For the fourth group (P4) semen was diluted egg yolk tris extender with NaCl 1,2% (placebo). Semen from each group (P1-P4) were processed into frozen semen. After being thawed, the percentage of motile and live spermatozoa were calculated. Data was analyzed using Analysis of variance followed by Tukey test. In conclution the addition of 48 ug tyrosine kinase in a very low concentration did not increase the percentage of motile and live spermatozoa of thawed semen.
Keyword :
Motility, Live Spermatozoa, Holstein Friesian, Extender, Tyrosine Kinase,
References :
Madyawati, S.P.,(2007) Suplementasi Tirosin Kinase Spermatozoa Sapi Friesien Holstein (FH) Terhadap Kualitas Semen Beku Surabaya : [Desertasi]. Universitas Airlangga
Arifiantini, R.I. dan T.L. Yusuf.,(2004) Keberhasilan Penggunaan Tiga Pengencer dalam Dua Jenis Kemasan pada Proses Pembekuan Semen Sapi Frisien holstein. . : http.www.google.com. [30 mei 2010].
Suyadi,(2003) Pengenceran Semen Kambing Dengan Beberapa Pengencer Sederhana dan Aplikasinya Untuk Insiminasi Buatan Malang : Penerbit Simetrika22
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