UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 8 / No. 2 / Published : 2015-07

Order : 2, and page :117 - 124

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Original Article :

The effects of insulin transferin selenium (its) supplementation on embryo freezing media at morula stage towards the percentage of embryonic development at blastula stage

Author :

  1. Cita Kristianti*1
  2. Widjiati*2
  3. Epy Muhammad Luqman*3
  4. Kuncoro Puguh Santoso*4
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan
  4. Dosen Fakultas Kedokteran Hewan

Abstract :

The aim of this research was to evaluate the influence of Insulin Transferin Selenium (ITS) Supplementation on embryo freezing media at morula stage towards the percentage of embryonic development at blastula stage. The experimental animals used female mice strain Balb C which were development at blastula stage. The experimental animals used female mice strain Balb C which were superovulated by using Pregnant Mare Serum Gonadotropin (PMSG) and human Chorionic Gonadotropin (hCG), afterwards monomatting was carried out. Three days after vaginal plug examination, the mice were sacrificed by conducting dislocatio cervicalis, next the uterus was removed and flushed. The morula embryos were classified into three groups : without ITS, ITS with 5μg/ml and 10μg/ml of concentration. Each treatment group was put into ministraw 0,5 ml and stored into liquid nitrogen for a week then thawed immediately. The post-thawed embryos were cultured by using tissue culture medium for 24 hours. Based on the statistical test, it demonstrated that there were no significant differences between the treatment group p>0,05with Chi square value of 1,144. Nevertheless, if it was investigated based on the percentage calculation of the blastula embryo development, it proved that 10μg/ml of ITS could increase the percentage of morula embryo development into blastula by 54% compared to 37% without ITS. In conclusion, based on statistical analysis, the distribution of Insulin Transferin Selenium (ITS) supplementation concentration on freezing media had not functioning optimally in increasing the morula embryos development into blastula after being cultured.

Keyword :

Vitrification, Insulin Transferrin Selenium, Embryo,


References :

Rimayanti,(2005) Pengaruh proses vitrifikasi dengan krioprotektan terhadap daya hidup oosit sapi 21(1) : Media Kedokteran Hewan

Widjiati, S. E. Pusporini dan M. Z. Arifin,(2012) Perbandingan angka fertilitas dan hambatan perkembangan embrio mencit yang dikultur dalam medium M16 dan Human Tubal Fluid 13 (3): 227-234 : Jurnal Veteriner





Archive Article

Cover Media Content

Volume : 8 / No. : 2 / Pub. : 2015-07
  1. The Effect Of Acupuncture On Sodium, Potassium, And Chloride Blood Plasma In Thoroughbred Horse After Exercise
  2. The Effects Of Insulin Transferin Selenium (its) Supplementation On Embryo Freezing Media At Morula Stage Towards The Percentage Of Embryonic Development At Blastula Stage
  3. The Liver Histopathology Of Mice (mus Musculus) Cause Exposure Carbofuran Insectiside
  4. Effect Of Mangosteen Rind Extract (garcinia Mangostana L.) To Necrosis Alveolar Type Ii Cell Of Lungonpregnant Mice (mus Musculus)exposured By Cigarette Smoke
  5. Histopathological Appearance Of Gastrointestinal Tract Iv Instar Mosquito Larvae Of Aedes Aegypti After Dipping With Active Compounds Of Permot Leaf Extract (passiflora Foetida Linn.) And Its Potential As Bioinsecticide
  6. The Effects Of Differences In The Duration Of The Thawing Of Frozen Semen To Sperm Quality Peranakan Ettawa Goat
  7. The Seminal Plasma Protein Of Cow Sustain Quality Of Beans Goat Spermatozoa After Equilibration In Freezing Process
  8. The Potential Of Garlic (allium Sativum) Infuse To Caecum Of Broilers Histopathology Infected By Escherichia Coli
  9. The Effectiveness Of Giving Haemopoetic Stem Cells In The Pregnant Mice (mus Musculus) Exposed To Plumbum Towards The Decreasing Number Of Necrocis Cells And Expression Of Caspase-3 In Placenta
  10. Protective Effect Of Binahong Leaf Extract [anredera Cordifolia (ten.) Steenis] To Renal Histopathological Of Rat (rattus Norvegicus) Induced With Borax (na2b4o710h2o)
  11. Effect Of Antibacterial Traditional Apple Vinegar And Apple Vinegar Products On The Growth Of Staphylococcus Aureus In Vitro
  12. The Effect Of Mangosteen Peel Extract (garcinia Mangostana L.) To The Number Of Placental Trophoblast Cells In Mice (mus Musculus) Exposure To Cigarette Smoke
  13. Analysis Of Humoral Immune Response (igg) In Rabbit Immunized With Proteins Of Leucocytozoon Caulleryi
  14. Isolation Of Methicillin-resistant Staphylococcus Aureus (mrsa) In Dog Nasal Swab With Oxacillin Screen Agar Confirmation Test
  15. Effect Of Prostaglandin F2α, Pregnant Mare Serum Gonadotropin, And A Combination Of Pregnant Mare Serum Gonadotropin And Human Chorionic Gonadotropin On Pregnancy Rates And Litter Size Of Mice
  16. Through The Provision Of Drinking Water Multiprobio Against Performan Broiler Chicken Production

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