UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 9 / No. 2 / Published : 2016-07

Order : 2, and page :105 - 112

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Original Article :

The effects of insulin transferin selenium supplementation on mice (mus musculus) embryos vitrification media at morula stage towards the viability of blastomere cells using fluorescence techniques after warming

Author :

  1. Didi Yudha Prawira*1
  2. Widjiati*2
  3. Arimbi*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

This research aimed to evaluate the influence of Insulin Transferin Selenium (ITS) supplementation on mice (Mus musculus) embryos vitrification media at morula stage towards the viability of blastomere cells using fluorescence techniques after warming. The experimental animals used were female mice strain Balb C which were superovulated using Pregnant Mare Serum Gonadotropin (PMSG) and Human Chorionic Gonadotropin(hCG), subsequently monomatting was carried out. Seventeen hours after vaginal plug examination, the mice were sacrificed by cervicalis dislocation, next the tuba fallopii was removed and fertilization pockets were torn. In Vitro Fertilization was done, then cultured for 72 hours to become morula stages. The morula embryos were classified into four groups: without ITS, ITS with 5 μg/100 ml, 10 μg/100 ml, and 15 μg/100 ml of concentration. Each treatment group was put into 0,25 ml ministraw and stored into liquid nitrogen for a week then warmed immediately. The post-warmed embryos were colored using fluorescent mark (Hoechst and Propidium Iodide) and cultured for 30 minutes. Observation of the viability of blastomere cells for morula ombryos was done using fluorescent microscope. Based on the statistical analysis, it demonstrated that there were no significant differences between the treatment group p>0,05, but significant differences between P3 (15 μg/100 ml) and without ITS group p<0,05. Nevertheless, if it was investigated based on the viability calculation of the blastomere cells morula embryo development, it proved that 15 μg/100 ml of ITS could increase the viability of blastomere cells morula embryo by 63% compared to 16% without ITS. In conclusion, the distribution of Insulin Transferin Selenium (ITS) supplementation concentration on vitrification media had been functioning optimally in increasing the viability of blastomere cells morula embryos after being warmed.

Keyword :

Vitrification, Insulin Transferin Selenium, Morula, Fluorescence,


References :

Batan, I. W., I. W. Suatha, W. E. Prasetyoningtyas, N. Handayani, I. Djuwita, dan A. Boediono,(2009) Vitrifikasi blastosis mencit dengan metode kriolup 10 (4) : 219-226. : Jurnal Veteriner

Choi, K., J. Kim, W. Gyung, Kim dan C. Chulhee,(2009) Oxidative stress-induced necrotic cell death via mitochondira-dependent burst of reactive oxygen species 6 (4) : 213-222 : Current Neurovascular Research

Das, Z. C., M. K. Gupta, S. J. Uhm and H. T. Lee,(2013) Supplementation of insulin–transferrin–selenium to embryo culture medium improves the in vitro development of pig embryos 18 :1-8 : Cambridge Journals Online (Abstract)





Archive Article

Cover Media Content

Volume : 9 / No. : 2 / Pub. : 2016-07
  1. Therapy Effect Of (pandanus Conoideus Inn) On Female Mice (mus Musculus) Infertility Model Toward The Folicle Diameter Size Of Pre Antral And Antral Histopathological Features
  2. The Effects Of Insulin Transferin Selenium Supplementation On Mice (mus Musculus) Embryos Vitrification Media At Morula Stage Towards The Viability Of Blastomere Cells Using Fluorescence Techniques After Warming
  3. Staphylococcus Aureus Contamination Rate On Beef Slaughter Results In Pegirian Surabaya Slaughterhouse
  4. Response Of Antibodies Formed As Result Of Brucella Suis Infection In Mice (mus Musculus) With Complement Fixation Test (cft)
  5. Therapeutic Effect Of Spirulinaplatensis Extract On Histopathological Appearance Of Ethanol Induced Liver Injury In Rat (rattus Norvegicus)
  6. Detection Gene Coding Capsule Type A Of Pasteurella Multocida From Cases Of Fowl Cholera By Polymerase Chain Reaction
  7. The Resistance Of Escherichia Coli From Pig Suspect Colibacillosis In A Pig Farm In Loa Duri Ulu Village To Antibiotics
  8. The Prevalence Of Bacteria Escherichia Coli On Meat Of Horse Slaughtered At Tumpang Abattoir In Malang District East Java
  9. Utilization Of Coconut Shell Liquid Smoke As Preservation In Chicken Intestine On The Value Of Total Plate Count
  10. Effect Of Red Fruit (pandanus Conoideus Lam.) Oil To Litter Size And Congenital Defecton Lead Intoxicated Pregnant Mice (mus Musculus)
  11. Sensitivity Test Of Some Antibiotic Types On Bacteria Of Endometritis In Pig Farm, Sukapura, Probolinggo
  12. Potential Test Of Cucumber Tree(averrhoa Bilimbi) Leaves Suspension As Alternative Mackerel (scomberomorus Sp) Preservatives
  13. Potention Of Alkaloid Sambiloto (andrographis Paniculata L) To Total Leukocytes And Leukocytes Differential Count On Rat Before Infected By Salmonella Typhimurium
  14. Influence Of Red Betel Leaf’s Extract (piper Crocatum) To The Histopatology Image On Rat’s Skin Which Infected By Staphylococcus Aureus
  15. Characteristic Morphology And Biochemical Of Cellulolytic Isolate Cytophaga Sp
  16. Red Cell Count (erythrocyte), Hematocrit, And Hemoglobin Of Catfish (clarias Gariepinus) After Infected By Aeromonas Hydrophila
  17. The Effect Of Extract Meniran (phyllanthus Niruri Linn.)to Ileum Histopathological Changes In Ratsinduced By Alcohol

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