UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 45 / No. 2 / Published : 2009-04

Order : 2, and page :93 - 96

Related with : Scholar   Yahoo!   Bing

Original Article :

14.5 kda protein of plasmodium falciparum is a specific asexual stage antigen recognized by pooled of mouse immune sera

Author :

  1. Heny Arwati*1
  2. Kusmartisnawati *2
  3. Yoes Prijatna Dachlan*3
  1. Department of Parasitology Airlangga University School of Medicine and Tropical Disease Center Airlangga University Surabaya, Indonesia
  2. Department of Parasitology Airlangga University School of Medicine and Tropical Disease Center Airlangga University Surabaya, Indonesia
  3. Department of Parasitology Airlangga University School of Medicine and Tropical Disease Center Airlangga University Surabaya, Indonesia

Abstract :

The genetic mutation in malaria parasite strain causing diversity characteristic of each strain. Many of the antigens, that P. falciparum express during their life cycle, particularly the asexual blood-stages, are antigenically diverse. The two major causes of antigenic diversity are allelic polymorphisms and antigenic variation. Each stage has different antigens that lead to protective immunity and in many cases; these antigens are not expressed at other stages of the life cycle. The TS.Ag isolated from P. falciparum 2300 strain containing mature asexual stages of parasite. Characterization of this antigen by SDS-PAGE and western blott methods has been performed to find out a malaria vaccine candidate for Indonesia locally. Comparison of the protein content of TS.Ag with R.Ag (ring form stage antigen) and RTS.Ag (ring form, trophozoite and schizont antigen) showed that, 14.5 kDa protein present in these three antigens. Western blott analysis of these antigens showed that, only 14.5 kDa protein of TS.Ag and R.Ag were recognized by mouse polyclonal antibody specific to P. falciparum asexual stage antigen, but not by pooled of malaria falciparum-infected human sera. The 14.5 kDa protein was similar to that of P. falciparum merozoit surface protein-8 (MSP-8) located on the parasitophorous vacuole of ring form and schizont stages of parasite.

Keyword :

Plasmodium falciparum asexual stage antigen, specific protein,


References :

Hoffman, S,L. and Miller, L. H.,(1996) A Perspective on Malaria Vaccine Development. In Hoffman, S. L. (ed.). Malaria Vaccine Development. A multi-immune Response Approach Washington DC : ASM Press

Dachlan, Y.P.,(2007) Localizatio of Plasmodium falciparum asexual stage antigen by mouse immune sera - : Folia Medica Indonesiana





Archive Article

Cover Media Content

Volume : 45 / No. : 2 / Pub. : 2009-04
  1. Correlations Between Hip Abductor’s Performance With Hip Bone Mineral Density In Elderly Women
  2. 14.5 Kda Protein Of Plasmodium Falciparum Is A Specific Asexual Stage Antigen Recognized By Pooled Of Mouse Immune Sera
  3. The Expression Of Mitochondrial Heat Shock Protein 10 In Oral Lesions With Hpv Positive
  4. Nosocomial Infection Monitoring Using Urine Catheter
  5. Pattern Of Community Role Development As An Effort To Increase Tuberculosis Patients Coverage With Passive Case Finding
  6. Effectiveness Of Cigarette Butts As An Alternative Material For Forensic Dna Identification With Polymerase Chain Reaction (pcr) In Short Tandem Repeat (str) Loci
  7. Effect Of Friction Technique Massage For Leg After Maximal Exercise On The Removal Rate Of Blood Lactate
  8. Physiological Effects Of Music During Exercise Secretion Of Hormones Cortisol And Endorphins
  9. Expression Of P53 Oncoprotein In Human Ductal Breast Carcinoma (in Situ, Invasive And Metastatic)
  10. Review Article : Congenital Heart Disease. The Holistic Approach, Now And  In The Future In Indonesia
  11. Review Article: Management Of Subclinical Ventricular Dysfunction
  12. Review Article: Exercise And Brain Health In Elderly
  13. Review Article And Clinical Experience: Emerging Multiple Properties Of High Dose Thiamine And B6-b12 Vitamins Therapeutic Possibilities For Diabetic Vascular Complications

Information index.php/JIPK/tmp_tumb/FMI