Folia Medica Indonesiana
ISSN 0303-7932
Vol. 48 / No. 4 / Published : 2012-10
Order : 5, and page :167 - 173
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Original Article :
Antibacterial effect of strawberry (fragaria x ananassa) extract on pathogenic serotype 1-11 escherichia coli revealed using dilution method
Author :
- Togar Erkasan Sitorus*1
- Sri Purwaningsih*2
- Manik Retno Wahyunitisari*3
- Mahasiswa Fakultas Kedokteran
- Dosen Fakultas Kedokteran
- Dosen Fakultas Kedokteran
Abstract :
Diarrhea is a clinical symptom of digestive (intestinal) disorders characterized by repeated and increased frequency of defecation more than usual, accompanied by a change in fecal form and consistency, which becomes soft or liquid. One way to deal with diarrhea is to kill the bacteria that cause diarrhea. Escherichia coli is one example of germs that cause diarrhea. Escherichia coli is a rod-shaped gram-negative bacteria. Although in our body Escherichia coli is obtained as normal flora, but there are some strains of Escherichia coli that are pathogenic to humans and able to cause diarrhea. One of the strains that can cause diarrhea in humans is pathogenic E. coli serotypes 1-11. Strawberries (Fragaria x ananassa) or also called strawberry orchard fruit is cultivated worldwide because of its aroma, bright red color and distinctive sweetness. Besides, strawberries are also shown to have a lot of content, including potassium, magnesium, phosphorus, calcium, iron, and vitamins A, E, K, C. Some studies also demonstrated that strawberries also have a flavonoid compound, one of which is antibacterial. The purpose of this study was to determine the antibacterial effects of strawberries (Fragaria x ananassa) extracts against pathogenic bacteria Escherichia coli serotype 1-11 in vitro. The research was conducted at the Laboratory of Medical Microbiology, Faculty of Medicine, University Airlangga on December 2011. Preparation of materials used extract methods without solvent, which then produced strawberry (Fragaria x ananassa) extracts in concentration of 100%. Then, we tested the extract against pathogenic bacteria Escherichia coli serotype 1-11 conducted with dilution method to search for bacteriostatic effects, then proceeded to the planting on solid media (plate) to prove the bactericidal effect. Results showed that bacteriostatic effect (minimum bacteria-inhibitory concentration) and bacteriocidal effect (minimum bacteria-killing concentration) were not found in strawberries (Fragaria x ananassa) extracts at all concentrations (100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.5625%) against pathogenic bacteria Escherchia coli serotype 1-11. (FMI 2012;48:167-173)
Keyword :
Strawberry, Fragaria x ananassa, pathogenic Escherichia coli serotype 1-11, dilution method sensitivity test,
References :
Departemen Kesehatan RI,(2000) Parameter Standar Umum Ekstrak Tumbuhan Obat Jakarta : Dirjen Pengawasan Obat dan Makanan Direktorat Pengawasan Obat Tradisional
Steel RGD and Torrie JH,(1989) Prinsip dan Prosedur Statistika Jakarta : Gramedia Pustaka
Archive Article
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