UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 49 / No. 1 / Published : 2013-01

Order : 6, and page :26 - 32

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Original Article :

Characterization of fertilin beta protein of human sperm membrane. a candidate for immunocontraception

Author :

  1. Reny I’tishom*1
  2. Doddy M. Soebadi*2
  3. Aucky Hinting*3
  4. Umie Lestari*4
  1. Department of Medical Biology Department, Faculty of Medicine, Airlangga University, Surabaya
  2. Department of Urology, Faculty of Medicine, Airlangga University, Surabaya
  3. Department of Medical Biology Department, Faculty of Medicine, Airlangga University, Surabaya
  4. Department of Biology, State University of Malang, Malang

Abstract :

In Indonesia, male family planning participants reached 1.3% of total 58.3% of all participants. So far, there are only two kinds of male contraceptions, the use of condoms and vasectomy. Condoms are effective in preventing pregnancy by 75-80%, whereas vasectomy is permanent. We need new male contraceptive with long-term benefits, but reversible in causing azoospermia. Contraceptive vaccine is a new concept to control fertility. Injected immunocontraception is an alternative of immunological contraception using antigenic material to prevent the meeting between spermatozoa and oocytes. One potential candidate is spermatozoa antigen. One of these antigens is protein fertilin beta of human spermatozoa membrane, which is more secure, with high specificity, effective, practical, with less side effects and high probability of acceptance. The aims of this study were to isolate and identify protein fertilin beta of human sperm membrane. This exploratory laboratory study collected sperm and analyzed semen, undergoing immunocytochemystry, human spermatozoa membrane proteins separation with sonification and centrifugation, protein molecular weight determination of fertilin beta sperm membrane using SDS-PAGE electrophoresis, protein concentration measurements with the Biuret method, and determination of protein concentration fertilin beta, Western Blot was used to determine the fertilin beta specificity, and fertilin beta isolation with electroelusion techniques. It was found that human sperm membrane proteins results of SDS-PAGE profiles of protein bands had four bands with molecular weights of 17, 35, 75 and 140. These bands were subjected to Western blotting and fertilin beta protein revealing a molecular weight (MW) of 75 kDa with higher levels of 2407 ug/ml. (FMI 2013;49:26-32)

Keyword :

human sperm membrane, fertilin beta, immunocontraception,


References :

Aulanni’am,(2004) Prinsip dan Teknik Analisis Biomolekul, cetakan pertama Malang : Fakultas Pertanian Universitas Brawijaya Press

Bohring C, Krause E, Habermann B, Krause W,(2001) Isolation and identification of sperm membrane antigens recognized by antisperm antibodies, and their possible role in immunological infertility disease - : Mol Hum Reprod





Archive Article

Cover Media Content

Volume : 49 / No. : 1 / Pub. : 2013-01
  1. The Effect Of Emotional Quality Management On Cortisol, Tnf-, And Il-2 In Women With Breast Cancer
  2. Reduced Addiction In Drugs Abusers Undergoing Dhikr At Ponpes Inabah Xix Surabaya
  3. Induced Abortion At Midgestation Of Sheep By Stress
  4. Polymerase Chain Reaction Of Mycobacterium Tuberculosis Gyrb Gene Region For Rapid Screening Test Of Pulmonary Tuberculosis
  5. Muscle Glucose Transporter 1 (glut-1) Expression In Diabetic Rat Models
  6. Characterization Of Fertilin Beta Protein Of Human Sperm Membrane. A Candidate For Immunocontraception
  7. Il-2 Level In Diabetic Mice Due To Obesity Is Higher Than That In Healthy Mice
  8. Supportive Pediatric Programs At Kampai Tabu Karambia Community Health Center Towards Mdgs 2015
  9. Correlation Between Superoxide Dismutase Serum And Sensoryneural Hearing Disorder In Patients With Multi Drug Resistance Tuberculosis
  10. Ethanol Extract From Petiveria Alliacea (singolawang) Inhibits The Growth Of Mycobacterium Tuberculosis In Vitro
  11. Acceleration Of Desa Siaga Aktif In Subdistrict Bareng, Jombang, 2012
  12. Effect Of Rimpang Temu Giring (curcuma Heyneana Val. & V. Zijp.) And Rimpang Temu Hitam (curcuma Aeruginosa Roxb.) Boiled Water On The Mortality Of Fasciola Hepatica Worm In Vitro
  13. Cytological Diagnostic Accuracy In Skin Tumor

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