UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 43 / No. 2 / Published : 2007-04

Order : 1, and page :64 - 67

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Original Article :

Detection of mycobacterium leprae dna in blood of the subclinical leprosy

Author :

  1. Cita Rosita Sigit Prakoeswa*1
  2. Indropo Agusni*2
  3. Shinzo Izumi*3
  1. Department of Dermato-Venereology
    Airlangga University School of Medicine, Dr Soetomo Teaching Hospital
  2. Department of Dermato-Venereology
    Airlangga University School of Medicine, Dr Soetomo Teaching Hospital
  3. Airlangga University Tropical Disease Centre, Surabaya, Indonesia

Abstract :

Subclinical Leprosy (SL) is attributed to healthy individuals living in endemic leprosy area, without any clinical signs of leprosy, but showing a high specific antibody level to leprosy bacilli. This laboratory finding indicates that a certain amount of Mycobacterium leprae (M. leprae) have entered the body and induces specific antibody. Due to the potential progress from SL to overt (manifest) leprosy, the management of SL is important in Leprosy Control Program. The aim of this study was to detect M. leprae DNA in the blood of SL cases, using Polymerase Chain Reaction (PCR) technique. Two ml peripheral blood samples from 29 SL cases diagnosed by clinical examination and serological test (ELISA : IgM anti PGL-1 antibody > 600 u/ml) were collected. DNA extraction was performed using Takara technique. Lp1 – Lp4 nested primers was used in PCR to amplify the RLEP sequence (99 bp) that is specific and sensitive for M. leprae. After running in the electrophoresis field, the results were observed by UV transilluminator. Results revealed that from 29 blood samples of SL cases, 6 (20.69 %) showed positive in PCR test. No significant differences the level of anti PGL-1 antibody was observed between positive and negative PCR results. In conclusion, M. leprae DNA can be found in peripheral blood of SL cases. This finding supports the opinion that SL cases need anti-leprosy treatment to prevent progression towards manifest leprosy.

Keyword :

Mycobacterium leprae, DNA, subclinical leprosy,


References :

I Agusni,(2001) Kusta stadium subklinis dan kedudukannya dalam epidemiologi penyakit kusta no info : MKI

MI Bakker,(2002) Epidemiology of leprosy on five isolated islands in the Flores Sea Indonesia no info : Trop Med Int Health

JI Cartel,(1990) Assessment of Anti PGL-1 IgM levels using ELISA for detection of Mycobacterium leprae infection in population of South Pasific Island no info : Int J Lepr

HD Donoghue,(2001) PCR primers that can detect low levels of Mycobacterium leprae DNA no info : J Med Microbiol

J Holton,(2001) PCR primers that can detect low levels of Mycobacterium leprae DNA no info : J Med Microbiol

M Spigelman,(2001) PCR primers that can detect low levels of Mycobacterium leprae DNA no info : J Med Microbiol

JT Douglas,(2004) Prospective study of serological conversion as a risk factor for development of leprosy among household contacts no info : Clin and Diagnostic Lab Imm

DN Durrheim,(2003) Global leprosy elimination no inf



Archive Article

Cover Media Content

Volume : 43 / No. : 2 / Pub. : 2007-04
  1. Detection Of Mycobacterium Leprae Dna In Blood Of The Subclinical Leprosy
  2. Correlation Between Mutant P53 Protein Expression And Histopathological Grading In Astrocytoma Patients
  3. Histopathological Feature Of The Tumor Of Central Nervous System At Airlangga University School Of Medicine, Dr Soetomo Hospital, Surabaya, In 2001-2003
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  11. Review Article And Clinical Experience: Hypertension In Diabetic Nephropathy. The Five “satan Circles”, Dha-licoar, The Roles Of Valsartan

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