UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 44 / No. 4 / Published : 2008-10

Order : 5, and page :251 - 254

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Original Article :

Difference in the productivity of b-lymphocyte isolated from lymph nodes and that isolated from mice spleen in the production of toxocara cati monoclonal antibody using hybridoma technology

Author :

  1. I Ketut Sudiana*1
  1. Airlangga University School of Nursing, Mulyorejo, Surabaya

Abstract :

Monoclonal antibody is highly required, particularly in diagnostic development. The antibody can be produced through hybridoma technology. Hibridoma is a result of fusion between myeloma and B-lymphocytes that has been sensitized by an immunogene. Today, the B-lymphocyte used for producing hybridoma is the one isolated from spleen. However, the obtained formation of hybridoma is remarkably less. One method to increase the productivity of monoclonal antibody production is by using B-lymphocyte isolated from lymph nodes. The difference in productivity between B-lymphocyte isolated from lymph nodes and that isolated from spleen remains unclear. This was an experimental study using 32 culture cells, in which myeloma cells were fused with B-lymphocyte from lymph nodes and that from spleen of mice that had been sensitized with embryonated egg of T. cati intraperitoneally. Obtained results revealed significant difference (p < 0.05), either in the number of formed hybridoma or its capability to produce antibody, between hybridoma resulting from fusion of myeloma and B-lymphocyte isolated from lymph nodes and that from isolated from spleen. In conclusion, B-lymphocyte isolated from lymph nodes is more productive than that isolated from mice spleen as the pair of myeloma cells in the production of monoclonal antibody using hybridoma technology.

Keyword :

hybridomas, B-lymphocyte, lymph nodes, spleen,


References :

Wheater, PR, Burkitt, HG, Daniel, VG,(1987) Functional Histology, 2nd edn Edinburgh : Churchill Livingstone

Hudson, P, Irving, B, Hoogenraad, N, Hillyard, C,(1992) A new era in immunodiagnostics - : J Today’s Life Science

Kohler, G,(1981) The technique of hybridomas production - : Immunol Meth





Archive Article

Cover Media Content

Volume : 44 / No. : 4 / Pub. : 2008-10
  1. Genetic Variation Of G6pd And Adaptive Phenomena In Malarial Infection
  2. Molecular Analysis Of The Coding Sequence Of Mage-1 Gene From Hepatocelular Carcinoma Patient And Testis
  3. Potential Value Of C-reactive Protein As Inflammation Marker And Il-1β, Tnf- As Pro-inflammatory Cytokine On Disease Activity Of Rheumatoid Arthritis
  4. Cell Surface Her2/neu Oncoprotein Expression In Breast Ductal Carcinoma
  5. Difference In The Productivity Of B-lymphocyte Isolated From Lymph Nodes And That Isolated From Mice Spleen In The Production Of Toxocara Cati Monoclonal Antibody Using Hybridoma Technology
  6. Exploration In The Use Of Medicinal Plant Acanthus Ilicifolius L In The Treatment Of Endothelial Dysfunction
  7. Detection Of M. Tuberculosis And Drug Sensitivity Test From Sputum Culture Of Pulmonary Tuberculosis Patients In Dr Soetomo General Hospital Between 2003-2004
  8. The Pharmacokenetics Of Levofloxacin In Healthy Rabbit Treated With Antacid
  9. Potency Of Lactobacillus Reuteri On Reduced Inflammation In Septic Encephalopathy
  10. Pharmacokinetic Parameters Determination Of A Single 500-mg Oral Dose Of Levofloxacin In Healthy Subjects By Using Blood Data
  11. Encephalopathy And Neuroendocrine Disorder In Septic Shock
  12. Osteoarthritic Pain Treatment With Electroacupuncture
  13. Review Article And Clinical Experience: Astaxanthin – Oxidative Stress – Diabetes Mellitus From Basics To Clinics And From General To Specific

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