UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 45 / No. 1 / Published : 2009-01

Order : 8, and page :41 - 44

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Original Article :

Hyaluronan, tnf-α and estradiol concentrations in infertile women with endometriosis

Author :

  1. Hendarto H*1
  2. Hetharia FP*2
  3. Moeloek FA*3
  4. Soetjipto*4
  5. Prabowo P*5
  1. Dept. of Obstetric & Gynecology School of Medicine University of Airlangga/Dr Soetomo Hospital Surabaya
  2. Dept. of Obstetric & Gynecology School of Medicine University of Airlangga/Dr Soetomo Hospital Surabaya
  3. Dept. of Obstetric & Gynecology School of Medicine University of Indonesia/Cipto M Hospital Jakarta
  4. Lab. of Biochemistry School of Medicine University of Airlangga
  5. Dept. of Obstetric & Gynecology School of Medicine University of Airlangga/Dr Soetomo Hospital Surabaya

Abstract :

Endometriosis is defined as the presence of endometrial-like tissue outside the uterus, which induces a chronic inflammatory reaction.  The association between endometriosis and infertility has not yet been clarified but one of the suspected factors is the abnormality of oocyte development. The higher concentration of hyaluronan has been identified as crucial factor for abnormality of oocyte maturation. Estradiol has been correlated with oocyte development. The purpose of this study was to clarify the concentrations of hyaluronan, TNF-alpha and estradiol in infertile women with endometriosis. Using a cross sectional study, peritoneal and follicular fluid were aspirated laparospically from 38 infertile women in mid cycle and divided into 3 groups: non endometriosis, mild and severe endometriosis in tertiary fertility clinic. Elisa laboratory technique was performed to all samples to detect peritoneal fluid TNF-alpha, estradiol and follicular fluid hyaluronan concentrations. In control, mild and severe endometriosis groups, the concentrations of hyaluronan were as follows: 954.14 ± 294.87; 1499.29 ± 131.49 and 1786.77 ± 44.64 pg/ml , TNF-alpha : 124.09 ± 99.28; 244.03 ± 106.51 and 409.08 ± 231 pg/ml , estradiol : 298.13 ± 131.37; 567.98 ± 368.82 and 750.50 ± 181 pg/ml, respectively. There were significant difference concentrations of hyaluronan, TNF-alpha and estradiol between 3 groups (p<0.05).  The correlations were found among TNF-alpha, estradiol and hyaluronan, but the significant correlation was only found between estradiol and hyaluronan (p<0.05). In conclusion, the increase concentration of hyaluronan in endometriosis might be correlated with the abnormality of oocyte maturation. The inflammatory and hormonal milieu play a role in the abnormality of oocyte maturation in infertile women with endometriosis, but more study is needed. 

Keyword :

hyaluronan, TNF-alpha, estradiol, endometriosis, infertility,


References :

Bedaiwy MA, Falcone T, Sharma RK, Goldberg JM, Attaran M, Nelson DR, Agarwal A,(2002) Prediction of endometriosis with serum and peritoneal fluidmarker: a prospective controlled trial - : Human Reproduction

Carlberg M, Nejaty J, Froysa B, Guan Y, Soder O, Bergqvist,(2000) Elevated expression of tumour necrosis factor a in cultured granulosa cells from women with endometriosis - : Human Reproduction





Archive Article

Cover Media Content

Volume : 45 / No. : 1 / Pub. : 2009-01
  1. Comparison Of Acid-base Balance Change In Hypovolemic Shock With 0.9% Normal Saline Therapy And Ringers Lactate. A Controlled Randomized Clinical Test
  2. The Effectiveness Of Acid Phosphatase And Zinc Tests On The Seminal Fluid Spot Examination As The Primary Identification. A Laboratory Observational Study
  3. The Relationship Between The Width And That Of Dental Arch Of The Upper Jaw In Patients With Down’s Syndrome Which Is Measured By Anthropometric Methods
  4. Immuno-modulatory Effect Of Probiotic And Sublingual Immunotherapy In Asthmatic Children
  5. Difference Of Plasma Interleukin-10 Level In Pulmonary Tuberculosis Patients And Healthy Nurses With Pulmonary Tuberculosis Risk
  6. Isolation Of Male Antifertility Compound In N-butanol Fraction Of Justicia Gendarussa Burm. F. Leaves
  7. Lps Helicobacter Pylori Causative Agent Of The Increase Of Tnf-α, Vcam, E-selectin And Mmp-8 As Indicator Of Endothelial Dysfunction
  8. Hyaluronan, Tnf-α And Estradiol Concentrations In Infertile Women With Endometriosis
  9. Factors Affecting Compliance To Medication In Lung Tuberculosis Patients
  10. Antiseizure And Neurotoxicity Effects Of Ligustrosid Glycoside Isolated From Fraxinus Graffithii Clarke Compared To Phenytoin And Diazepam
  11. Effect Of Andrographis Paniculata Extract On Mammary Carcinoma In Rats Induced By Dmba
  12. Review Article: Ultrasound Elastography: Development Of The Imaging Technique For Tissue Elasticity Assessment
  13. Review Article And Clinical Experience: Thyroid Storm: Quick Diagnosis And Quick Treatment. The Roles Of Formulas G/o–p-39.5 – 120 And Cs-7.1.5

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