UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 43 / No. 1 / Published : 2007-01

Order : 8, and page :39 - 43

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Original Article :

Localization of plasmodium falciparum asexual stage antigen by mouse immune sera

Author :

  1. Heny Arwati*1
  2. Sukmawati Basuki*2
  3. Bariah Ideham*3
  4. Sri Hidajati*4
  5. Kusmartisnawati*5
  6. Ani Safriah*6
  7. Luki Enggar Fitri*7
  8. Yoes Prijatna Dachlan*8
  1. Department of Parasitology Airlangga University School of Medicine Surabaya, Indonesia
  2. Department of Parasitology Airlangga University School of Medicine Surabaya, Indonesia
  3. Department of Parasitology Airlangga University School of Medicine Surabaya, Indonesia
  4. Department of Parasitology Airlangga University School of Medicine Surabaya, Indonesia
  5. Department of Parasitology Airlangga University School of Medicine Surabaya, Indonesia
  6. Department of Parasitology Airlangga University School of Medicine Surabaya, Indonesia
  7. Department of Parasitology Airlangga University School of Medicine Surabaya, Indonesia
  8. Department of Parasitology Airlangga University School of Medicine Surabaya, Indonesia

Abstract :

Malaria is still a major health problem in the world as well as the increased incidence of this disease in the tropical regions. In Indonesia, rapid people movement from/to Java Island is one of reasons in increasing malaria incidence. The use of imprecise dose of antimalarial drugs for medication in community is causing mutation in the parasite genes. This is resulting in parasite resistance to antimalarial drugs. Genetic variation of P. falciparum affects the diversity of clinical symptoms, pathology, transmission characteristic, and human response to antimalarial drugs. An alternative to overcome this problem is using vaccine. However, due to those reasons and also due to the complexity of parasite life cycle the effective and global vaccine is difficult to produce. Through this research we are trying to develop a malaria vaccine for being applied to Indonesia locally. This research is an early step of the main research in malaria vaccine development. This current research is to characterize Plasmodium falciparum asexual stage antigen in order to find out a malaria vaccine candidate for local Indonesia. Previously, 5 BALB/c mice have been immunized with asexual stage antigen of P. falciparum 2300 strain and Freund complete adjuvant. Sera were collected every single week and used to localize the P. falciparum asexual stage antigen by means of indirect immunofluorescent assay (IFA). The results showed that, sera from 3 and 4 weeks post immunization recognized the antigen. Antigens were localized on the surface of late trophozoite, early and late schizont stages, and on the surface of internal and external merozoites. Mouse sera did not recognize ring form and trophozoite stages. It meant that, there was no contamination with ring form and early trophozoite stages during antigen preparation. Hemozoin or malaria pigment was recognized neither by mouse nor by human sera, due to the present of Fe++ in hemozoin.

Keyword :

Plasmodium falciparum, asexual stage antigen,


References :

RF Anders,(1991) Antigenic diversity in Plasmodium falciparum no info : Acta Leiden

H Arwati,(2004) Reactivity of Plasmodium falciparum-infected human sera from several endemic areas of Indonesia to falciparum merozoites antigen Surabaya : Airlangga University

C Bumi,(2004) Reactivity of Plasmodium falciparum-infected human sera from several endemic areas of Indonesia to falciparum merozoites antigen Surabaya : Airlangga University

H Arwati,(2005) 116 kDa and 21 kDa. Plasmodium falciparum asexual stage antigen recognized by falciparum-infected human sera from several endemic areas of Indonesia Bandung : National Seminar on Medical Parasitology and Entomology

K Berzins,(1996) Malaria vaccines: attacking infected erythrocytes Washington DC : ASM Press

P Perlmann,(1996) Malaria vaccines: attacking infected erythrocytes Washington DC : ASM Press

HD Engers,(1998) Malaria vaccine development: current status no info : Parasitol Today

T Godal,(19



Archive Article

Cover Media Content

Volume : 43 / No. : 1 / Pub. : 2007-01
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  8. Localization Of Plasmodium Falciparum Asexual Stage Antigen By Mouse Immune Sera
  9. Description Of Urinary Retention In Women A Retrospective Study In Dr Soetomo Hospital Surabaya
  10. Review Article: The Potential Intervention On Apoptosis And Cell Cycle Activity In Rheumatoid Arthritis
  11. Review Article: The Role Of Microglia As Prime Component Of Cns Immune System In Acute And Chronic Neuroinflammation
  12. Review Article And Clinical Experience: A Novel “two-in-one” Drug Fixed Combination Of Ros And Met Its Roles On Ir, Prediabetes, Mets, T2dm And Cvd

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