UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 44 / No. 2 / Published : 2008-04

Order : 4, and page :82 - 85

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Original Article :

Pcr technique detection of chlamydia trachomatis dna band using silver nitrate staining

Author :

  1. Rebekah J Setiabudi*1
  2. Manik Retno W *2
  3. Nurul Wiqoyah*3
  1. Department of Microbiology, Airlangga University School of Medicine, Jl. Prof Dr Moestopo 47, Surabaya
  2. Department of Microbiology, Airlangga University School of Medicine, Jl. Prof Dr Moestopo 47, Surabaya
  3. Department of Microbiology, Airlangga University School of Medicine, Jl. Prof Dr Moestopo 47, Surabaya

Abstract :

Chlamydia trachomatis genital infection is one of the Sexually Transmitted Diseases that can cause cervicitis, salphingitis, ectopic pregnancy, infertility, chronic pelvic pain and pelvic inflamatory disease (PID) in women and epididymitis and non gonococcal urethritis (NGU) in men. The prevalence of this infection is 7.9% in women and 7.6% in men overall. The problem is that 70% of this infection in women and 50% in men are asymptomatic and can only be detected through accurate screening test. The nucleotide amplification using PCR technique could be used to amplify one DNA or RNA molecule of the microbe specific gene region that may increase the sensitivity and specificity to detect Chlamydia trachomatis in the “first-catch urine”. The aim of this research was to study the accuracy (sensitivity and specificity) of the PCR with Silver Nitrate staining method in comparison to the commercial PCR (Amplicor, Roche) as a standard method. The result was no significant difference (p < 0.05) found in accuracy between PCR Silver Nitrate staining and PCR Amplicor; the sensitivity of PCR Silver Nitrate staining was 85.7% and the specificity was 100%, to detect Chlamydia trachomatis in the “first-catch urine”.

Keyword :

Chlamydia trachomatis, urine, PCR, silver nitrate, screening test,


References :

Black, CM,(1997) Current Methods of Laboratory Diagnosis of Chlamydia trachomatis Infections - : J Clin Microbiol

Sambrook, J, Fritsh, E, Maniatis, T,(1989) Molecular Cloning Laboratory Manual, 2nd edn - : Cold Spring Harbour Laboratory Press

Verkooyen, RP, Noordhoek, GT, Klapper PE, Reid, J, Schirm, J, Cleator, GM et al,(2003) Reliability of nucleic acid amplification methods for detection of chlamydia trachomatis in urine: Results of the First International Collaborative Quality Control Study among 96 Laboratories - : J Clin Microbiol





Archive Article

Cover Media Content

Volume : 44 / No. : 2 / Pub. : 2008-04
  1. Tgf-β1 As The Trigger Of Pulp Fibroblast Proliferation
  2. Difference In Cyclooxygenase-2 (cox-2) Expression Between Low And High Grade Papillary Carcinoma In Human Bladder Cancer
  3. Detection Of Cytokeratin 19 Mrna In Blood As An Early Marker Of Micrometastatic Tumor Cells Of Nasopharyngeal Carcinoma Patients In Surabaya
  4. Pcr Technique Detection Of Chlamydia Trachomatis Dna Band Using Silver Nitrate Staining
  5. Change Of Cd4 Count, Hsp70 And Anti-hsp70 Levels In Hiv Patients
  6. Hiv/aids Prevalence In Surabaya, Indonesia
  7. Treatment Response And Acute Toxicity Evaluation Of 70 Gray Radiation In Nasopharyngeal Carcinoma Patients In Dr Soetomo Hospital, Surabaya
  8. Pharmacodynamic Effects Of Ligustroside Glycoside (a Cns Active Substance) Isolated From Fraxinus Graffithii Clarke Against Chemically Induced Seizures On Mice
  9. Management Of Acute Glomerulonephritis In A Child With Ischemic Stroke And Hypertensive Crisis
  10. Efficacy Of Orlistat Plus Diet Compared To Diet Alone In Reducing Body Weight And Body Mass Index
  11. Review Article: Pathogenesis Of Repeated Abortion In Polycystic Ovarian Syndrome (pcos)
  12. Review Article: Protein Calorie Malnutrition And Immune Response In Children
  13. Review Article And Clinical Experience: Varenicline (champix®) : A Breakthrough For Smoking Cessation Treatment (an α4ß2 Nicotinic Acethylcholine Receptor Partial Agonist)

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