Folia Medica Indonesiana
ISSN 0303-7932
Vol. 49 / No. 3 / Published : 2013-07
Order : 1, and page :134 - 138
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Original Article :
Pharmacodynamics study of cholecalciferol to glut4 protein translocation in muscle fiber of hyperglycemia micewhich inducted by streptozotocin
Author :
- Elly Nurus Sakinah*1
- Student of Magister Program in Medical Sciences Faculty of Medicine, Airlangga University
Abstract :
Glucose uptake into skeletal muscle cells require insulin-dependent and insulin independent signaling pathways, both leading to thetranslocation of glucose transporter-4 (GLUT4) to the plasma membrane. Insulin resistance occurs due to failure of insulin signalingto translocate GLUT4 resulting in the failure of glucose uptake and causing hyperglycemia. Cholecalciferol is known to have afunction in regulating calcium homeostasis was shown to increase the synthesis of insulin and increasing insulin sensitivity. Thepurpose of this study is to explain the role of cholecalciferol to increased GLUT4 translocation in skeletal muscle cells. 30 miceadapted for one week and then induced using 150mg/kg BW streptozotocin (STZ) intraperitoneally, after experiencing hyperglycemiamice were divided into 5 groups (n=6 each), Group I (hyperglycemic control), group II (25ng cholecalciferol), group III (50ngcholecalciferol), group IV (100ng cholecalciferol), and group V (metformin 300mg/kgBBB). Cholecalciferol given orally for 14thdays. On day 15th the mice were sacrificed so then the gastrocnemius muscle tissue can be taken for immunohistochemicalexamination using rat GLUT4 polyclonal antibodies. The assesment of protein GLUT4 was conducted by quantifying ImmunoReactive Score (IRS-GLUT4). Based on statistical analysis showed that there were significant differences in the number of cells ofskeletal muscle GLUT4 translocation between groups (p<0,001). Based on univariate regression analyses there was significantdoses response relationship (p<0,001). Cholecalciferol may increase GLUT4 translocation in skeletal muscle cell membranes inhyperglycemia mice models.(FMI 2013;49:134-138)
Keyword :
Cholecalciferol, GLUT4, skeletal muscle, streptozotocin,
References :
Calle C, Maestro B, Arencibia MG,(2008) Genomic actions of 1,25-dihydroxyvitamin D3 on insulin receptor gene expression, insulin receptor number and insulin activity in the kidney, liver and adipose tissue of streptozotosin-induced diabetic rats - : BMC Molecular Biology
Ceglia L and Harris S,(2013) Vitamin D and its role in skeletal muscle - : Calcif Tissue Int
Archive Article
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 Volume : 49 / No. : 3 / Pub. : 2013-07 |
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