UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 43 / No. 1 / Published : 2007-01

Order : 1, and page :1 - 6

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Original Article :

Serum p53 protein and total lymphocyte profile in sepsis patients treated with ciprofloxacin and cefotaxime

Author :

  1. Agus Yudho Santosa*1
  2. Nasronudin*2
  3. Eddy Soewandojo*3
  1. Balung Hospital, Balung, Jember
  2. Department of Internal Medicine Airlangga University School of Medicine Dr Soetomo Teaching Hospital, Surabaya
  3. Department of Internal Medicine Airlangga University School of Medicine Dr Soetomo Teaching Hospital, Surabaya

Abstract :

Sepsis is still the main cause of death in the hospital. The frequency of cefotaxime resistant bacteria is increasing. On the other hand, cefotaxime has a potency to stimulate a high LPS release. Ciprofloxacin is an alternative antibiotic agent against sepsis with low LPS release characteristics. Higher LPS release is associated with the increase of lymphocyte apoptosis. The mechanism of cell death (apoptosis), especially lymphocytes, is through the expression of serum p53 protein. The objective of this study was to determine serum p53 protein and total lymphocyte profile of patients with sepsis treated with ciprofloxacin and cefotaxime. This research was an observational study. The effect of ciprofloxacine versus cefotaxime on concentration of p53 protein and total lymphocyte in the sera of patients with sepsis was evaluated. This study included 50 patients suffering sepsis caused by bacteria, treated with either ciprofloxacine or cefotaxime after thorough clinical and microbial evaluation and followed up for sera of p53 protein and total lymphocyte. Serum p53 protein and total lymphocyte from the ciprofloxacin group and cefotaxime were analyzed on the first, sixth, and ninth days of treatment. Data were presented descriptively to determine the profile of serum p53 protein and total lymphocyte. From all of the patients, 40 (80%) cured and 10 (20%) died. In cefotaxime group, 19 (28%) cured and 6 (12%) died. In ciprofloxacin group, 21 (42%) cured and 4 (8%) died. The average serum p53 protein in cured patients showed a decrease as compared to normal value (<0.2 U/ml) either in ciprofloxacine or cefotaxime group. The average serum p53 protein of ciprofloxacin in the cured group were 2.23 U/ml, 1.36 U/ml, 0.75 U/ml on the first, sixth, and ninth day. The average serum p53 protein in the cured cefotaxime group on the first, sixth and ninth day were 6.14 U/ml, 4.09 U/ml, and 3.08 U/ml respectively. Average total lymphocyte in cured patients showed alteration. The average total lymphocyte in cured patients showed an increase as compared to normal limit (2000/UI) in both groups. The average total lymphocyte in cured ciprofloxacin group on the first, sixth, and ninth day were 15243/uL, 2007.8/uL, and 2309.3/uL respectively, and that in cured cefotaxime group were 1287.4/uL, 2006/uL, and 2095.6/uL. The average total lymphocyte in cured patients during treatment increased both in the ciprofloxacin (1524.3/uL) and the cefotaxime group (1287.4/uL). In conclusion, there was a tendency of decreased serum p53 protein and increased total lymphocytes in sepsis patients who was treated either with ciprofloxacin or cefotaxime. Based on this study, serum p53 protein and total lymphocyte may have potential indicators for prognosis in sepsis patient.

Keyword :

serum p53 protein, total lymphocyte, sepsis,


References :

CR Bone,(1992) Definition for sepsis and organ failure and guide lines for the use of innovative therapies in sepsis no info : Chest

RA Balk,(1992) Definition for sepsis and organ failure and guide lines for the use of innovative therapies in sepsis no info : Chest

FB Cerra,(1992) Definition for sepsis and organ failure and guide lines for the use of innovative therapies in sepsis no info : Chest

A Burke,(2002) Antibiotic essensials New York : Physicians Press

Cunha,(2002) Antibiotic essensials New York : Physicians Press

W Gardjito,(2002) Rational use of antibiotics in the management of sepsis Surabaya : PIT I Perhimpunan Patobiologi Indonesia

A Hanafiah,(2002) Rancangan Percobaan Aplikasi dan Teori Jakarta : Pusat Penerbitan FKUI

RS Hotckhiss,(2003) The pathophysiology and treatment of sepsis no info : NEJM

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IE Karl,(2003) The pathophysiology and treatment



Archive Article

Cover Media Content

Volume : 43 / No. : 1 / Pub. : 2007-01
  1. Serum P53 Protein And Total Lymphocyte Profile In Sepsis Patients Treated With Ciprofloxacin And Cefotaxime
  2. Effect Of Loranthaceae Daendropthoe Species Infusion On Serum Transaminase Level And Hepatic Necrosis In Rats
  3. Glucose 6 Phosphate Dehydrogenase (g6pd) Deficiency In Bad Pregnancy History In St. Vincentius A Paulo Catholic Hospital, Surabaya
  4. Heparin Effect On Vegf And Egfr After Deep Second And Third Degree Burns In Rattus Novergicus Strain Wistar
  5. Examination Of Oxldl Serum Level As The Prognosis Of Acute Thrombotic Ischemic Stroke
  6. Mental Changes In Parkinson's Disease A Study Of Fifty Patients
  7. Microscopic Picture And Water Content In Dentin Analysis Of Bonding Agent Bond To Dentin Surface An Evaluation Of Various Humidity
  8. Localization Of Plasmodium Falciparum Asexual Stage Antigen By Mouse Immune Sera
  9. Description Of Urinary Retention In Women A Retrospective Study In Dr Soetomo Hospital Surabaya
  10. Review Article: The Potential Intervention On Apoptosis And Cell Cycle Activity In Rheumatoid Arthritis
  11. Review Article: The Role Of Microglia As Prime Component Of Cns Immune System In Acute And Chronic Neuroinflammation
  12. Review Article And Clinical Experience: A Novel “two-in-one” Drug Fixed Combination Of Ros And Met Its Roles On Ir, Prediabetes, Mets, T2dm And Cvd

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