Folia Medica Indonesiana
ISSN 0303-7932
Vol. 42 / No. 4 / Published : 2006-10
Order : 4, and page :218 - 227
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Original Article :
The effect of melanin concentration on collagen accumulation in keloid
Author :
- David S Perdanakusuma*1
- Department of Plastic Surgery Airlangga University School of Medicine Dr Soetomo Teaching Hospital, Surabaya
Abstract :
This research arises from the revelation that colored-skin people have a high incidence of keloid, in contrast to albinos who never experience this entity. Keloid is an abnormal scar, characterized by collagen accumulation. Melanin is the most important pigment determining skin color variations in humans. The correlation between increased melanin concentration and collagen accumulation was assumed to be linked to pH. An experimental laboratory research was performed, employing the principle of biochemical reaction between collagen and collagenase, to observe pH in various concentrations of melanin, and collagen-collagenase reactions under various pH and in different concentrations of melanin (n=73). A cross sectional observational research was performed on keloid , evaluating tissue pH, melanin concentration and collagen concentration (n=30). Research data were analyzed using t-test and regression statistical methods. The results of this research demonstrated that there was a significant difference between the results of absorbance under pH≤7.2 and pH>7.2 (p<0.05). Melanin concentration increased in proportion to its decreased pH (p<0.05). Decreased pH led to decreased collagen degradation (p<0.05). Increased melanin concentration gave no effect on collagen degradation (p>0.05), due to the effect of assay buffer used. All keloid tissues possessed a pH of ≤7.2. Melanin increased in proportion to its decreased pH (p<0.05). Decreased pH caused increased collagen concentration (p<0.05). Increased melanin concentration led to increased collagen concentration (p<0.05). Increased melanin concentration and decreased pH progressively increased collagen concentration (p<0.05). This research concludes that melanin plays a role in collagen accumulation by decreasing the pH that eventually disrupts collagen degradation process.
Keyword :
keloid, melanin, pH, collagen synthesis, collagen degradation, collagenase,
References :
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GF Crickelair,(1961) The surgical treatment of keloids no info : Plast Reconstr Surg
DM Ju,(1961) The surgical treatment of keloids no info : Plast Reconstr Surg
JC Gaulin,(1961) The surgical treatment of keloids no info : Plast Reconstr Surg
R Lattes,(1961) The surgical treatment of keloids no info : Plast Reconstr Surg
B Cosman,(1977) Scars and keloids Philadelphia : WB Saunders Company
DMC Ju,(1977) Scars and keloids Philadelphia : WB Saunders Company
GF Crikelair,(1977) Scars and keloids Philadelphia : WB Saunders Company
DD Datubo-Brown,(1990) Keloid: a review of the literature no info : Br J Plast Surg
IMP Dawson,(1982) Theory and Practice of Hystologycal Techniques no info : Churchill Livingstone
A Eisen,(1968) Human skin collagenase, isolation and mechanism of attack on the collagen the colagen molecule no info : Biochim Biophy
Archive Article
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 Volume : 42 / No. : 4 / Pub. : 2006-10 |
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