Folia Medica Indonesiana
ISSN 0303-7932
Vol. 45 / No. 1 / Published : 2009-01
Order : 2, and page :6 - 9
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Original Article :
The effectiveness of acid phosphatase and zinc tests on the seminal fluid spot examination as the primary identification. a laboratory observational study
Author :
- Hoediyanto*1
- Department of Medicolegal & Forensic Medicine, Airlangga University School of Medicine, Dr Soetomo Teaching Hospital, Jl Prof Dr Moestopo 6-8, Surabaya
Abstract :
Spermatozoa in the vaginal tract are a definite sign of intercourse. However, there are times during microscopic examinations that none of the spermatozoa are detected or it turns out that the tests are false negative. One of the many factors that can affect this circumstance is no ejaculation in the vaginal tract. In addition, there are also other factors, such as oligo/azoospermia, vasectomy, time-degeneration of sperm, wrong sample taking, and improper storage. Therefore, it is necessary to have examinations on the other ejaculate components which are acid phosphatase, choline and spermine enzymes. The value of verification is lower when phosphatase, choline or spermine enzymes are compared to spermatozoa, because these three components are less specific. However, the concentration of phosphatase enzyme in the vaginal tract is lower compared to the seminal fluid from prostate gland. From this study, 192 samples had been taken from sperms/semen-spotted cloths, and were examined with acid phosphatase and zinc tests by using direct and indirect methods. Rinsing was performed in the first method on the first and seventh day and the third month, where each was later examined. Whereas the second method had rinsing and tests ran together on the first and seventh day and the third month. Rinsing was performed with seven different brands of detergents on four different sperm spot samples and also with water as a control. The result of the study showed that sensitivity value was lower (0.186) while the specificity was high. This showed that both tests had high specificity, where specific acid phosphatase test revealed the presence of phosphatase enzyme, while specific zinc test showed zinc contain in the semen. Phosphohydrolase-phosphate enzyme was easily disintegrated due to external factors such as temperature, humidity and also chemical substances, such as Sodium Douducyl Sulphate (SDS) that has the ability to catalyze the enzymes. The weakness of the acid phosphatase test is the result of the characteristics of an enzyme, which is easily disintegrated, partially or totally, due to the external factors such as temperature, humidity, heat and chemicals.
Keyword :
effectiveness, acid phosphatase and zinc tests, identification, seminal spot,
References :
Eckert, WG,(1980) Introduction to Forensic Sciences St. Louis : The CV Mosby Co.
Hafes, ESE,(1976) Human Semen and Fertility Regulation in Men St. Louis : The CV Mosby Co.
Archive Article
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 Volume : 45 / No. : 1 / Pub. : 2009-01 |
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