UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 47 / No. 3 / Published : 2011-07

Order : 5, and page :166 - 170

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Original Article :

The role of glucose and phosphate in in vitro culture medium to overcome cell block on mouse embryo

Author :

  1. Widjiati*1
  2. Y. Sukra*2
  3. B. Purwantara*3
  4. I Djuwita*4
  1. Faculty of Veterinary Medicine, Airlanggga University
  2. Faculty of Veterinary Medicine, Bogor Agricultural University
  3. Faculty of Veterinary Medicine, Bogor Agricultural University
  4. Faculty of Veterinary Medicine, Bogor Agricultural University

Abstract :

The influence of phosphate and/or glucose on the development of mice preimplantation embryos was investigated. Approximately two hundreds female mice (Mus musculus albinus) CBR strain 2 month of age were used. The animal were superovuleted using PMSG and HCG and were bred naturally by adult males (3-4 month of age). The one cell stage of embryos were harvested and used for culture in modified media supplemented with 0,0 mmol/l, 0,5 mmol/l, 1,0 mmol/l, phosphate and 0,0 mmol/l, 2,5 mmol/,l 5.0 mmol/l, 7.5 mmol/l, glucose. he results showed that supplementation of 1,0 mmol/l, phosphate support preimplantation embryos dewelopment better than of 0,5 mmol/l, and 1.5 mmol/l, supplementation of 2.5 mmol/l, glucose indicated to overcome cell-block, but the requirement of glucose to be increasing as the development more beyond two cell stage interaction of phosphate and glucose at 1.0 mmol/l, and 2.5 mmol/l, respectively support preimplantation mice embryos development. In conclusion, phosphate supplementation did not inhibited the development of embryos and cell-block can be overcome by lower concentrate of glucose the combination of phosphate and glucose serve a better environment for embryos development.

Keyword :

Cell Block, glucose, phosphate, mouse embryo,


References :

Aurich C and J Han,(1994) In vitro maturation, fertilisazation and culture of bofine oocyte in a modified menezo B2 Medium - : An Reprod Sci

Flood MR and JL Webold,(1988) Glucose metabolisme by preimplantation pig embryos - : J. Reprod. Fert

Kim J, Funahashi HH, Niwa K, Okuda K,(1993) Glucose requirerement at different developmental stages of in vitro fertilized bovine embryos cultured in semi difined medium - : Theriogenology

Laura CH, Martin KL Leese HJ,(1996) Effect of metabolic Inhibitors on mouse preimplantation embryos development an the energy metabolism of isulated inner cell masses - : j Mol Reprod and Dev

Miyoshi K, Funahashi H, Okuda K, Niwa K,(1994) Development of rat one-cell embryos in a chemically defined modium effects of glucose, phosphate and osmolarity - : J of Reprod and Fertil





Archive Article

Cover Media Content

Volume : 47 / No. : 3 / Pub. : 2011-07
  1. Lung Function Is Declined In Smoking “becak” Drivers
  2. The Increase Of Ehsp 72 In Members Of Dzikir Group
  3. Effectiveness Of Nuclear Dna Mini Primer Set At Loci Fga, Csf1po & D21s11 In High-temperature Dna Degradation With Polymerase Chain Reaction (pcr) Method
  4. The Effect Of Passive Smoking On The Incidence Of Primary Dysmenorrhea
  5. The Role Of Glucose And Phosphate In In Vitro Culture Medium To Overcome Cell Block On Mouse Embryo
  6. Prevalence Of Hepatitis B, Hepatitis C And Human Immunodeficiency Virus Infection Among Pediatric Hematology Oncology Patients With Repeated Transfusion
  7. Clinical Features Of Influenza A (h1n1) In Children At Dr Soetomo General Hospital
  8. Case Report: Pain Relieved In Acute Breast Infection Using Extra Anatomy Pathway
  9. Case Report Growth Hormone Treatment In Short Children. A Report Of 4 Cases
  10. Review Article: Flail Chest Management In Ards
  11. Review Article: Adequate Management Of Depression And Neuropathic Pain In Patient With Type 2 Diabetes

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