UNIVERSITAS AIRLANGGA



Detail Article

Jurnal Ilmiah Perikanan & Kelautan

ISSN 2085-5842

Vol. 6 / No. 2 / Published : 2014-01

Order : 1, and page :109 - 115

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Original Article :

Identification of koi herpes virus at different dose with streptavidin biotin methods immunohistochemistry on tilapia (oreochromis niloticus)

Author :

  1. Faricha Risma Nurani*1
  2. Hari Suprapto*2
  3. Suwarno*3
  1. Mahasiswa Fakultas Perairan dan Kelautan
  2. Dosen Fakultas Perairan dan Kelautan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

KHV is a viral disease in goldfish and Koi Fish (Cyprinus carpio) is a highly contagious, infecting all ages or sizes of fish and aquaculture systems. This disease results in mortality between 80% - 100% of the total fish population, with an incubation period of between 1-7 days. KHV infection triggered by a drop in ambient temperature so it is referred to as a virus that attacks when cold (a cold virus). The disease attacks the temperature range of 18-28 ° C and can cause death. This virus attacks can occur at any age of the fish ranging in size from the seed to the parent. The most prominent clinical symptoms due to KHV infection is the sudden death 1-2 days after infection. Other clinical symptoms are necrosis of the gills, sunken eyes, bleeding at the gills, hemorraghe, excess mucus production in the body, and secondary bacterial infections or parasitic infestations.Streptavidin biotin immunohistochemical method was applied to the study of cells and tissues by staining imunostaining. Technique of determining the existence of (location) antigen (target protein) in tissue using antigen-antibody reaction that begins with histotenik procedure is the procedure of making tissue sections (histology). This method has high sensitivity and fast so that it can be applied to the detection of KHV antigen in tissues of fish Tilapia (Orechromis niloticus).This study aims to determine the presence of KHV antigen in tilapia gills after infection. The method used in this study is the experimental method. Dose of virus titer used was 1 ID50, ID50 10, 100 ID50, ID50 1000.The results showed that the Streptavidin Biotin immunohistochemical examination imunopatologi able to detect KHV virus Ag in the gill tissue of tilapia (Oreochromis niloticus) were infected with different doses. In all treatments showed on all the gills are KHV antigen is indicated by the presence of a golden brown color on the gill epithelium. Advised Streptavidin biotin immunohistochemistry test was applied to the detection of the presence or absence of fish-carp KHV carrier as a routine control program and control, including the prevention and outbreak of KHV in Indonesia, because of KHV were attacked in tilapia was persistent and did not show clinical symptoms

Keyword :

KHV, Histopathology, Immunohistochemistry,


References :

R. Damayanti ; Alfinus,(2009) Deteksi Antigen Virus Rabies Pada Jaringan Otak Dengan Metode Imunohistokimia x : Seminar Nasional teknologi Petrnakan dan Veteriner

A. Isnansetyo ; Kurniastuti,(1995) Teknik Kultur Phytoplankton dan Zooplankton Pakan Alami untuk Pembenihan Organisme Laut Yogyakarta : Kanisius

Hari Suprapto,(2011) Histologi Ikan Surabaya : Fakultas Perikanan dan Kelautan Universitas Airlangga





Archive Article

Cover Media Content

Volume : 6 / No. : 2 / Pub. : 2014-11
  1. Identification Of Koi Herpes Virus At Different Dose With Streptavidin Biotin Methods Immunohistochemistry On Tilapia (oreochromis Niloticus)
  2. Genetic Diversity Cythochrome B Of Sidat (anguila Bicolor) Assesed By Restriction Fragment Length Polymorhisme (rflp)
  3. Vertebrae Malformation Tilapia Fish (oreochromis Niloticus) On Different Media Hatching Saline
  4. The Isolation Development Of Chondroitin Sulphate From Cuttlebone (sepia Phraonis), Ray’s Cartilage (raja Sp.), And Shark (carcharinus Falciformes)
  5. Applications Of Addition Earthworms (lumbricus Rubellus) On Feed Of Sangkuriang Catfish (clarias Sp.)
  6. Monitoring Of Water Quality On Rearing Ponds Of Vannamei Shrimp (litopenaeus Vannamei) In Situbondo, Jawa Timur
  7. The Total Of Cellulolytic Bacteria In Gunung Anyar Surabaya And Bancaran Bangkalan Estuaries
  8. Water Quality Standards For Marine Life And Pollution Index In Cirebon Coastal Area In The Dry Season
  9. Effect Of Mung Bean Sprouts Essences Against Malondialdehyde Levels In African Catfish (clarias Gariepinus) That Was Infected By Bacteria Aeromonas Hydrophila
  10. Predilection And Anatomical Pathology Changes In Goldfish (carassius Auratus) Due To Lernaea Cyprinacea Infestation In Tulungagung
  11. Effect Of Rhizome Extract (kaempferia Galanga L.) For The Cure Rate Of Catfish Fry (clarias Sp) Infected By Saprolegnia Sp
  12. Antifungal Activity Test Of Basil Leaves Juice (ocimum Sanctum Linn.) Against Aspergilllus Terreus By In Vitro
  13. Effect Of Different Substrates On The Recirculation System For Local Sea Cucumber (phyllophorus Sp.) Physiological During Adaptation Period
  14. Exploration Of Brown Seaweed (phaeophyceae) Active Subtance As Aedes Aegypti Biolarvicides
  15. The Bioabsorbtion Effects Of Mangrove Avicennia Alba Against Ammonia (nh3)
  16. Analysis Of Heavy Metal Concentration Difference Value On Cadmium (cd) Against Seaweed (eucheuma Cottonii) In Pamekasan And Sumenep Seashore - Madura
  17. Study Of Heavy Metal Content Of Mercury (hg) And Prediction Content Of Methyl Mercury (ch3hg) On The Blood Shellfish’ (anadara Granosa) Organs In Sidayu And Banyu Urip District, Gresik, East Java
  18. The Effects Of Bandotan Leave’s (ageratum Conyzoides) Essential Oil Within Closed System Transportation Of Koi Carp (cyprinus Carpio).

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