UNIVERSITAS AIRLANGGA



Detail Article

Jurnal Oftalmologi Indonesia

ISSN 1693-2587

Vol. 7 / No. 3 / Published : 2010-06

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Original Article :

Effect of genistein on conjunctival goblet cells density and the expression of interleukin-1bin ovariectomized rats

Author :

  1. Ovi Sofia*1
  2. Rosy Aldina*2
  1. Department of Ophthalmology Faculty of Medicine, Brawijaya University, Dr. Saiful Anwar General Hospital Malang

Abstract :

The aim of this research is to evaluate the effect of genistein on conjunctival goblet cells density and IL-1b expression of ovariectomized(OVX) rats. Female adult Wistar rats were subjected to bilateral OVX and divided into 3 groups of genistein treatments (0.1 mg/kg/day,0.5 mg/kg/day, 1.0 mg/kg/day) subcutaneous (sc) and 3 groups of control, 2 groups of OVX rats without treatment as positive control,and 1 group of normal rats as negative control. At the end of procedures, right eyeball and eyelid was dissected. Periodic acid-Schiffstaining was performed in histologic sections to determine the numbers of goblet cells present. The expression of IL-1b was observedusing immunohistochemistry. The number of goblet cells in OVX rats as positive control was significantly decreased compared withnormal rats (p < 0.05). There were no significant differences of goblet cells number observed between negative control and all ofgenistein treatment groups (p > 0.05). There were significant increase of IL-1b expression on positive control groups compared withnormal (p < 0.05). Expression of IL-1b in treatment groups was not significantly different compared with negative control at the doseof 0.5 mg/kg/day and 1.0 mg/kg/day (p > 0.05). The higher conjunctival goblet cells density, the lower expression of IL-1b. Genisteinat the dose of 0.5 mg/kg/day sc can improve conjunctival goblet cells number and reduce expression of IL-1b of OVX rats. Goblet cellsdensity inversely correlated with expression of IL-1b

Keyword :

genistein, dry eye, ovariectomy, goblet cell, IL-1b, conjunctiva,


References :

Solomon A, Dursun D, Liu Z, Xie Y, Macri A, Pflugfelder SCSolomon A, Dursun D, Liu Z, Xie Y, Macri A,(2001) Pro- and anti-inflammatory forms of interleukin-1 in the tear fluid and conjunctiva of patients with dry-eye disease - : Invest Ophthalmol Vis Sci

Narayanan S, Miller WL, McDermott AM,(2006) Conjunctival cytokine expression in symptomatic moderate dry eye subjects - : Invest Ophthalmol Vis Sci

Gulati A, Dana R,(2005) Keratoconjunctivitis sicca: clinical aspects. In: Foster CS, Azar DT, Dohlman CH. Smolin and Thoft’s: The Cornea Philadelpia : Lippincott Williams & Wilkins





Archive Article

Cover Media Content

Volume : 7 / No. : 3 / Pub. : 2010-06
  1. Effect Of Genistein On Conjunctival Goblet Cells Density And The Expression Of Interleukin-1bin Ovariectomized Rats
  2. The Relation Of Onset Of Trauma And Visual Acuity On Traumatic Patient
  3. The Role Of Human Papilloma Virus Type 16 In Retinoblastoma
  4. Ocular Survival Rate Penderita Retinoblastoma Yang Telah Dilakukan Enukleasi Atau Eksenterasi Di Rsud Dr. Soetomo Surabaya
  5. Human Papilloma Virus In Progressive Pterygium And Stationary Pterygium
  6. Perbandingan Ekspresi P53 Pada Pterigium Primer Progresif Setelah Pemberian Injeksi Subkonjungtiva Dengan Mitomicin C Dan 5 Fluorourasil
  7. Effect Of Genistein On Conjunctival Goblet Cells Density And The Expression Of Interleukin-1b In Ovariectomized Rats
  8. Management Of Upper Eyelid Coloboma With Three Steps Technique Surgery
  9. Ptotic Management In Marcus Gunn Jaw-winking Syndrome
  10. The Caterpillars Eat One Eye On The Pseudo Lymphoma Tumor
  11. The Untreatable Case Of Retinopathy Of Prematurity Grade V

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