UNIVERSITAS AIRLANGGA



Detail Article

Jurnal Penelitian Medika Eksakta

ISSN 1411-6626

Vol. 8 / No. 1 / Published : 2009-04

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Original Article :

Profile and identify the serum of amiloid protein ( sap) as acute phase protein at mice which inoculated salmonella enteritidis

Author :

  1. Sunarni Zakaria*1
  2. Rochmah Kurnijasanti*2
  3. Iwan Sahrial Hamid*3
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

ABSTRACTThis research have a purpose to identify SAP as acute phase protein from mice which inoculated Salmonella enteritidis with the method SDS-PAGE. Its important is role SAP in detecting early case of infection and inflammation, as protektor of infectif agent and prevent the culture degradasi pass role antiproteinase, so that require to be isolation SAP. Used 80 mouse old age three months of mean weight 25 g, decided our treatment group, each treatment group consisted five mouse inokulasi with the bacterium of Salmonella enteritidis 106, 108, 109 and 1010 cell / ml by intraperitoneal. Later then at 20, 32, 38 and 44 hours after inoculated by removed blood serum, protein level rate from serum detected by chromatografi. Serum which have been collected is ready to weared to be identified with the Sodium of Dodecyl Sulfate Polyacrylamide of Gel Electroforesis ( SDS Page) to know the to molecular weight. Result have been identified by the serum protein from mouse of molecular weight equal to 25,89 kDa that suggest SAP. The Protein identified from mouse which infection of Salmonella enteritidis of equal to 106 and 108 at collection 20 and 44 hours after inoculation.

Keyword :

Amiloid protein, salmonella enteritidis,


References :





Archive Article

Cover Media Content

Volume : 8 / No. : 1 / Pub. : 2009-04
  1. The Differences Concentration Of Tooth Whitening Material Toward Cytotoxicity Using Mtt Assay
  2. Profile And Identify The Serum Of Amiloid Protein ( Sap) As Acute Phase Protein At Mice Which Inoculated Salmonella Enteritidis
  3. The Differences Concentration Of Tooth Whitening Material Toward Cytotoxicity Using Mtt Assay
  4. The Determination Of The Shelf Life Of Extemporanevs Carbanazepine Caapsule
  5. Chemical Composition And Activity Of Propolis As Anti Microbial From Malang East Java
  6. Histopatological Study Of Renal Rats (rattus Norvegicus) After Ovaryohysterectomized And Given High Dose Of Calcium Carbonate As Supplement
  7. Antibacterial Activities Of Sambiloto Herbs Extract (andrographis Paniculata Nees) On Staphylococcus From Milk Of Dairy Cows Mastitis
  8. The Exploitation Of Kutorejo Mojokerto Clay As Buffer Isolation Material For Industrial Product
  9. Identifikasi Enterotoksin Isolat Staphylococcus Aureus Dari Sarang Walet
  10. Analisis Faktor Untuk Mengetahui Sikap Bereisko Kecelakaan Pada Lanjut Usia (lansia)

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