UNIVERSITAS AIRLANGGA



Detail Article

Media Kedokteran Hewan

ISSN 2015-8930

Vol. 18 / No. 1 / Published : 2002-04

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Original Article :

Analysis of the chemically and freeze dried preserved complement titer stability

Author :

  1. Nanik Sianita*1
  2. Rahaju Ernawati*2
  3. Wahju Tjahjaningsih*3
  1. Laboratorium Virologi dan Imunologi, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya
  2. Laboratorium Virologi dan Imunologi, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya
  3. Laboratorium Virologi dan Imunologi, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya

Abstract :

The purpose of this study was about to know the preparative form of complements (complement preserved chemically and freeze dried) that influenced the complement titer at several storage temperatures (room temperature and 4 derajat Celcius) and prolong storaged. Preparation and preservation of complements which were collected from 30 rabbits sera had been measured to obtain the titer. After the complement had divided into 3 parts, one part for chemically preservation treated with 6 % Sodium Acetate, 2 % Boric Acid and 0,25 % Sodium Azide, second part for freeze drying and the third part was for control without any treatment. The stability of each complement titer was measured after some periods of storage (1, 2, 3, 6, 12, 24, 48 days) according to the modified microtechnique method. The results showed that preserved complement either chemically preserved and freeze dried had more stabled titer than non-preserved complement stored at room temperature and 4 derajat Celcius. The storage of preserved complements (chemically preserved and freeze dried) at 4 derajat Celcius for 48 days yielded a significant higher titer compared to room temperature storage. While the duration of storage influenced both the preserved complements titer at room temperature and 4 derajat Celcius.

Keyword :

complement, freezedried, chemically preserved,


References :





Archive Article

Cover Media Content

Volume : 18 / No. : 1 / Pub. : 2002-04
  1. The Effect Of Ultra Violet (uv) Radiation On Post Thawing Sperm Of Dairy Cattle Against Sex Ratio Of Sperm Cells Bearing Chromosome X And Y
  2. Equalion Models Of Progesterone And Estradiol Concentrations With Pregnancy Age In Ewes
  3. The Effect Of Antibodies Anti-inhibin In Obtaining Of Anti-idiotipic Antibodies On Mice
  4. The Separation Of Sex Bearing Chromosome Sperm And Embryo Development Produced Of In Vitro Fertilization Of Madura Cattle
  5. Chichen Embryo Fibroblast Cell Culture As An Media Hybrid B Cell Limphocyte And Mouse Myeloma Cell In Incubator Without Carbon-dioxyde
  6. Analysis Of The Chemically And Freeze Dried Preserved Complement Titer Stability
  7. Electron Microscope Scanning (ems) And Morphology Of The Some Species Of Haemonchus Sp. From Goat/sheep Slaughtered At Pegirian Slaughter House Surabaya
  8. Controlling Toxocariasis Infection On Calves In Madura Against Isolated The Volatile Oil From Curcuma Aeruginosa Roxb (temuireng)
  9. Analysis Of Restriction Fragment Length Polymorphisms For Molecular Epidemiological Tracing Of Escherichia Coli Strains Using Probes
  10. Morphofunction Evaluation Of Porcine Autogeneous Cephalic Vein Grafts In Arterial Circulation
  11. Estrogenicity Effect Test Of Piper Nigrum L. On Female Rat
  12. The Effect Of Frog Waste Meal In Feed On Abdominal Fat Percentage And Subcutan Fat Value Of Broiler Chicken
  13. Comparison Of Aeromonas Hydrophila Vaccination Method By Immersion And Hyperosmotic Infiltration Against The Antibody Titers

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