UNIVERSITAS AIRLANGGA



Detail Article

Media Kedokteran Hewan

ISSN 2015-8930

Vol. 17 / No. 3 / Published : 2001-12

Related with : Scholar   Yahoo!   Bing

Original Article :

Follicle size selection influences of chromosome transformation profile on goat oocytes in in-vitro maturation

Author :

  1. Widjiati*1
  2. Rimayanti*2
  3. Budiarto*3
  1. Lab. Fisiologi Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Airlangga, Surabaya.
  2. Lab. Kemajiran Veteriner Fakultas Kedokteran Hewan Universitas Airlangga, Surabaya.
  3. Lab. Kesehatan Masyarakat Veteriner Fakultas Kedokteran Hewan Universitas Airlangga, Surabaya.

Abstract :

This study was aimed to define the influence of follicle size on chromosome transformation level of goat oocytes. Folicle where divided into two groups, based on the criterion of follicle diameter large or smaller than 100 µm. Oocytes were maturated in TCM 199 with the addition of 5 µg/ml LH, 3 % BSA and 50 mg/ml gentamycine sulfate. After incubation for 22 hours, the oocytes were stained with 1 % aceto orcein. Results showed that the oocytes colected from follicle with diameter of less than 100 µm diameter reached GV, GVBD, M I, M II at 8 %, 64 %. 13 % and 15 % respectively. While the oocytes colected from large size follicles reached GVBD, M I, and M II at 24 %, 26 % and 50 % respectively. From these results it could be concluded that follicle with diameter large than 100 µm showed better maturations compared with those of follicles less than 100 µm.

Keyword :

follicle size, maturation, transformation chromosome,


References :





Archive Article

Cover Media Content

Volume : 17 / No. : 3 / Pub. : 2001-12
  1. The Effect Of Acepromazine Premedication On Intraocular Pressure In Dogs Anasthetized With Ketamine Hcl
  2. The Effect Multi Dosage Of Sulphadiazine On Histopathological Liver Of Mice (mus Musculus)
  3. Preliminary Study On The Isolation And Identification Of Yeasts In Horse Milks Distributed In Bandung Region
  4. Pregnancy Rate And Sex Ratio Offspring Resulted From Separation Of Y Spermatozoa With Column Albumin Methods
  5. Extremely Low Frequency Electromagnetic Field Effects On The Histologic Properties Of Rat Aorta
  6. The Number Trypanosoma Evansi In Mice Blood During The Acute And Chronic Phases
  7. Pregnancy Rate And Sex Ratio Offspring Resulted From Separation Of Y Spermatozoa With Column Albumin Methods
  8. The Use Of Progesterone Levels In Miik For Determination The Accuracy Of Timing Of Insemination In Dairy Cows
  9. The Effect Of Sperm Treatments Of Madura Cattle On Live Percentage, Motility And Cleavage Rate
  10. Follicle Size Selection Influences Of Chromosome Transformation Profile On Goat Oocytes In In-vitro Maturation
  11. The Effect Of Treatment Of Prophyltiouracyl On Male Rat Against Pregnancy Of White Female Rat

Information index.php/JIPK/tmp_tumb/MKH