UNIVERSITAS AIRLANGGA



Detail Article

Majalah Obstetri & Ginekologi

ISSN 0854-0381

Vol. 19 / No. 3 / Published : 2011-09

Order : 2, and page :102 - 108

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Original Article :

Expression of kit ligand and amount of follicles as features of folliculogenesis disorder on rat (rattus novergicus) strain wistar with cisplatin

Author :

  1. M. Ferri Komarhadi*1
  2. Hendy Hendarto*2
  3. Suhatno*3
  4. Widjiati*4
  1. Mahasiswa Fakultas Kedokteran
  2. Dosen Fakultas Kedokteran
  3. Dosen Fakultas Kedokteran
  4. Dosen Fakultas Kedokteran Hewan

Abstract :

Kit Ligand (KL), one of the first growth factors in ovarian follicle, plays a key role in mammalian oogenesis and folliculogenesis. KL protein and mRNA expression is detected in the granulosa cells of follicles at all stages of development. Cisplatin is one of the first generation platinium compounds and is commonly used for treatment of ovarian, breast, testicular, and bladder cancers.The objective of this study was to prove the lower expression of kit ligand and amount of follicles, and the association between the expression of kit ligand and the amount of follicles, on rat (Rattus novergicus) strain wistar with cisplatin. This study was conducted at Laboratory of Experimental Animal and Veterinary Patology, Faculty of Veterinary, Airlangga University, Surabaya, May-July 2011. The research was performed by randomized post test only control group design. The research samples were Rat (Rattus novergicus) strain wistar which was admitted into inclusion criteria. At the diestrus period, the control group was injected by NaCl 0.9% IP, and the cisplatin group was injected by cisplatin 5 mg/kg w IP. The rat were slaughtered at the 7th day of estrus period on the control group, and at the diestrus period on the cisplatin group. Oophorectomy was performed, continued with immuno- histochemistry staining and hematoxylin eosin for examination on expression of kit ligand and amount of follicles. We achieved no association among the expression of kit ligand and the amounts of primordial, primary, secondary, and tertiary follicles (p=0.945, p=0.180, p=0.590, p=0.753) on the rat with cisplatin. In conclusion, In rat with cisplatin, the expresssion of kit ligand, the amount of primordial, primary, secondary, and tertiary follicles are low.  (MOG 2011;19:102-108)

Keyword :

expression of kit ligand, amount of follicles, cisplatin, folliculogenesis, rat,


References :

Sonmezer M and Oktay K,(2006) Fertility Preservation in Female Patients - : Human Reproduction Update

Simon B, Lee SJ, Partridge AH and Runowicz CD,(2005) Preserving Fertility After Cancer - : Cancer J Clin





Archive Article

Cover Media Content

Volume : 19 / No. : 3 / Pub. : 2011-09
  1. The Effect Of Curcumin To The Anti Mullerian Hormone Serum Concentration In Endometriotic Rat Model
  2. Expression Of Kit Ligand And Amount Of Follicles As Features Of Folliculogenesis Disorder On Rat (rattus Novergicus) Strain Wistar With Cisplatin
  3. Comparison Of The Accuracy Of Pipelle Biopsy Method With The Method Of Dilatation - Curettage In Endometrial Tissue Sampling To Establish A Histopathologic Diagnosis In Patients With Hysterectomy At Dr Soetomo Hospital, Surabaya
  4. Hasil Pemeriksaan Mikroskopis Dan Pcr Malaria Dari Darah Tepi, Jaringan Plasenta Dan Darah Tali Pusat Pada Ibu Bersalin Yang Mendapat Terapi Act. Studi Operasional Pada Empat Kecamatan Di Kabupaten Indragiri Hilir Propinsi Riau
  5. Perbandingan Kadar Tnf- Dan Mikroba Patogen Periodontal Pada Ibu Hamil Normal, Hamil Dengan Periodontitis, Dan Preeklampsia Berat Tipe Lambat
  6. Karakteristik Penderita Kanker Serviks 2006-2010 Di Rsud Dr. Soetomo
  7. Differences In The Expression Of Hiv-1 Rna Of Infants Aged 3 To 18 Months Based On The Current Provision Of Antiretroviral Therapy In Pregnant Women With Hiv In Dr Soetomo Hospital Surabaya Between January 2004 To December 2011

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