UNIVERSITAS AIRLANGGA



Detail Article

OVOZOA

ISSN 2302-6464

Vol. 3 / No. 1 / Published : 2014-04

Order : 3, and page :156 - 160

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Original Article :

Dna fragmentation as apoptotic indicator and it relation with sperm viability and motility of domba ekor gemuk frozen semen in different thawing duration

Author :

  1. Rahmalia Dwi Suindarti*1
  2. Imam Mustofa*2
  3. Suherni Susilowati*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The aim of this study were to determine DNA fragmentation expression of Domba Ekor Gemuk (DEG) frozen semen and it relation with spermatozoa viability and motility in different thawing duration. Domba Ekor Gemuk frozen thawed in 37°C water  with 20, 40, 60 second respectively. DNA fragmentation expression was detected by using ApopTag® Plus Peroxidase In Situ Apoptosis Detection Kit. The positive result showed tan brown to black colour at the head region of spermatozoa. The result indicated that there was significant difference (p<0,01) between viability, motility, DNA fragmentation between the three thawing duration.

Keyword :

Frozen semen, thawed, DNA fragmentation, apoptosis,


References :

Lasso, J.L., Noiles, E.E., and Alvarez, J.G. ,(1994) Mechanism of superoxide dismutase loss from human sperm cells during cryopreservation. USA : J. Androl. 15: 255–265.

Salomon, S. and W.M. Maxwell. ,(2000) Storage of Ram Semen. USA : Anim Reprod Sci. 62:77-111





Archive Article

Cover Media Content

Volume : 3 / No. : 1 / Pub. : 2014-04
  1. Morning And Evening Serum Testosterone Level Of Limousine Crossbreed Bulls
  2. Test Potential Polyclonal Antibodies Local (abpo Local Pmsg) And Patents (abpo Patent Pmsg) Pmsg (pregnant Mare Serum Gonadotropin) From Male Rabbit (oryctolagus Cuniculus) To Pregnancy Mice (mus Musculus)
  3. Dna Fragmentation As Apoptotic Indicator And It Relation With Sperm Viability And Motility Of Domba Ekor Gemuk Frozen Semen In Different Thawing Duration
  4. Determination Encoding Genes Heat Stable Toxin (st) And Protein Of Pathogens Escherichia Coli Pili K99 Was Isolated From Dairy Cow Feces Using Polymerase Chain Reaction (pcr)
  5. Biopotensi Pmsg (pregnant Mare Serum Gonadotrophin) Local Horse To Speed Time Arising Estrus, Number Of Follicles, Corpus Luteum, Progesterone Levels And Pregnancy Rate In Madura Cattle
  6. Potential Of Vitamin E (α-tocopherol) Of Malondialdehyde (mda) Levels Testis Mice (mus Muscular) Induced 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (tcdd)
  7. Effect Of “sarang Semut” (myrmecodia Pendans) On The Number Of Spermatogenic Cells In Seminiferous Tubules Of Mice (mus Musculus) With Excessive Physical Treatment
  8. The Effect Of Estrous Syncronization By Pgf2α And Superovulation By Pmsg And Hcg And Artificial Insemination On Heat Response, Pregnancy Rates, Litter Size And Sex Ratio Of The Crossbread Etawa Goat
  9. Examination Of Fresh Semen Quality Of Merino Sheep For Frozen Semen Production In Teaching Farm Of Veterinary Medicine Faculty Of Airlangga University
  10. Identification Of Sperm Morphometry In Merino Sheep And Fat-tailed Sheep
  11. The Correlation Between Body Weight With Body Lenght, Wither’s Height, Heart Girth, Scrotal Circumference, Volume, And Semen Quality Of Peranakan Etawa Goat
  12. Profile Of Progesterone And Insulin-like Growth Factor-i (igf-i) In Pregnant Crossbred Mare Serum
  13. Effect Of Equilibration Time Extender To Post Thawing Motility And Viability Of Merino Sheep’s Sperms In Egg Yolk Skim Diluents
  14. The Effect Of The Combination Progesterone In Sponge Intravaginal With Equine Chorionic Gonadotropin (ecg) To The Occurrence Of Estrous On Fat Tailed Sheep

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