UNIVERSITAS AIRLANGGA



Detail Article

OVOZOA

ISSN 2302-6464

Vol. 2 / No. 2 / Published : 2013-10

Order : 1, and page :1 - 8

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Original Article :

Extract of annona muricata as egg yolk citrate diluent substitute for fat-tailed sheep sperm quality

Author :

  1. Wirda Rachmanda Anandita*1
  2. Suherni Susilowati*2
  3. Soeharsono*3
  1. Mahasiswa S1
  2. Departemen Reproduksi Veteriner Fakultas Kedokteran Hewan Unair
  3. Departemen Anatomi Veteriner

Abstract :

The selection of type for diluent on semen is an important aspect in the implementation of artificial insemination in order to increase the reproductive efficiency of fat-tailed sheep. This study was aimed to determine the effect of soursop (Annona muricata) extract as egg yolk citrate diluent substitute for fat-tailed sheep sperm quality. The results were obtained by using ANOVA (Analysis of Varian) continued with duncan’s multiple range test. Semen was divided into four diluents treatment,1:4 egg yolk citrate (EYC), 1:1 EYC:soursop, 1:2 EYC:soursop, and 1:4 soursop:citrate (SC). The results showed that the percentage of sperm viability on the fifth day showed a significant difference (p <0.05) that EYC (34.91 ± 1.792) against EYC soursop 1:1 (43.71 ± 1.615), EYC:soursop 1:2 (46.28 ± 1.569), and SC (31.34 ± 0.727). So did the percentage of motility on the fifth day 1:2 EYC soursop, 1:1 EYC soursop, EYC, and SC, respectively is 41.71 ± 1.112, 40.00 ± 1.290, 32.91 ± 1.754 and 29.31 d ± 0.999 with a significant difference (p <0.05) in each treatment. The percentage of plasma membrane integrity in EYC at 32.91 ± 1.754 significantly different from the other treatments that 1:1 EYC soursop (41.51 ± 1.672), 1:2 EYC soursop (44.17 ± 1.348), and SC (29, 31 ± 0.999). The conclusion of this study was the EYC, 1:1 EYC:soursop and 1:2 EYC soursop could increase the percentage of sperm quality until the fifth day with the highest percentage yield on 1:2 EYC:soursop.

Keyword :

fat-tailed sheep, egg yolk citrate, soursop (Annona muricata), sperm quality,


References :

Nugroho, A.W., T. Sardjito, R. Bijanti. ,(2008) Kulits Air Mani itik Manila (Cairina moschata) pada Berbagai Perbandingan Pengencer Air Kelapa Mud Plus Kuning Telur. Surabaya : Journal of Poultry Diseases. Volume 1. Nomer 1. Halaman 36-44.

Herdis, Yulnawati, dan M.A. Setiadi. ,(2003) Pemanfaatan sari buah melon sebagai media pengencer semen cair alternatif spermatozoa domba Garut. Bogor : Jurnal Sains dan Teknologi Indonesia. 5(5):126-131.





Archive Article

Cover Media Content

Volume : 2 / No. : 2 / Pub. : 2013-10
  1. Extract Of Annona Muricata As Egg Yolk Citrate Diluent Substitute For Fat-tailed Sheep Sperm Quality
  2. Effect Of Different Injection Time Of Pregnant Mare Serum Gonadotropin (pmsg) On The Onset Of Estrous Of Fat Tailed Ewes Synchronized Using Intravaginal Progesterone
  3. The Effect Of A Diluent Contained Lecithin On The Quality Post Thawing Carp’s (cyprinus Carpio) Spermatozoa
  4. Effect Of Glycerol In Skim Egg Yolk Diluent On The Freezing Of Goldfish Spermatozoa (cyprinus Carpio)
  5. The Effect Of Clove Tea ( Syzygium Aromaticum ) On Histopathological View Of Mouse (mus Musculus) Testis With Cigarette Smoke Exposured
  6. Extract Banana An As Alternative Fructose Toward A Quality Of Fat-tailed Shepp’s Sperm
  7. Fertilization Rate Of Merino Sheep Frozen Semen In Diluent Egg Yolk Tris And Egg Yolk Skim To In Vitro Fertilization
  8. Reproductive Efficiency Of Beef Cattle Resulted From Artificial Insemination In Wringin Subdistrict, Bondowoso District In 2012
  9. Artificial Insemination Program For Beef Cattle In Madura Island “targets, Realisation And Problems”
  10. Effect Of Infusum Salam Leaves (eugenia Polyantha) Testis Spermatogenic Cells Amount Of White Rats (rattus Norvegicus) Induced Alloxan
  11. The Effect Of Bitter Melon Fruit (momordica Charantia L.) Extract On Pregnancy Rate And Litter Size Of Mice (mus Musculus)
  12. The Effect Of Giving Protein Fertility Associated Antigen (faa) To Capacitation And Acrosome Reaction On Frozen Semen Simental Bull After Centrifugation
  13. Effect Of Seminal Plasm Of Cow On Goat’s Spermatozoa Motility And Viabilityin In Milk Diluter
  14. The Effect Of Caffeine In Washing And Capacitation Media Of The Goat Spermatozoa On Pregnancy Rates Of Local Goat

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