UNIVERSITAS AIRLANGGA



Detail Article

OVOZOA

ISSN 2302-6464

Vol. 3 / No. 1 / Published : 2014-04

Order : 10, and page :198 - 201

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Original Article :

Identification of sperm morphometry in merino sheep and fat-tailed sheep

Author :

  1. Rizky Indah Pristyana*1
  2. Rudy Sukamto*2
  3. Bambang Poernomo S.R.*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The aim of this research was to identificate the sperm morphometry of both Merino and Fat-tailed sheep. Semen samples were collected twice a week from four rams, divided into two  were Merino sheep (MER) and two were Fat-tailed sheep (DEG). Sperm morphometry examination was performed using NaCl-formaldehyde fixed samples through phase contrast microscope micrometer addition with oil emersion (1000×). Sperm morphometry characteristics were measured by Nikon motorized microscope Ci-E with NiS software after applying Eosin-Negrosin staining technique. Each sperm was measured for four primary spermatozoa head dimensional parameters length [L (µm)], width [W (µm)], (area [A (µm2)], perimeter [P (µm)], two flagell parameters middle piece [MP (µm)], principal piece [PP (µm)] and total sperm length (µm). Merino (MER) and Fat-tailed sheep (DEG) were identified: MER, sperm morphometry L= 8.90 ± 0.45 µm, W = 4.79 ± 0.20 µm,  A = 33.84 ± 1.57 µm2, P = 22.72 ± 0.72 µm, MP = 14.81 ± 0.54 µm, PP = 42.15 ± 2.00 µm, total sperm length = 65. 86 ± 1.75 µm. DEG sperm morphometry L= 8.76 ± 0.33 µm, W = 4.80 ± 1.70 µm,  A = 34.52 ± 1.43 µm2, P = 22.48 ± 0.70 µm, MP = 15.18 ± 0.60 µm, PP = 42.92 ± 1.35 µm, total sperm length = 66.87 ± 1.31 µm.

Keyword :

Merino sheep, fat-tailed sheep, sperm morphometry,


References :

Arifiantini, R.I., T. Wresdiyanti dan E.F. Retnani. ,(2006) Kaji Banding Morfometri Spermatozoa Sapi Bali (Bos Sondaicus) menggunakan Pewarnaan Williams, Eosin, Eosin Negrosin dan Formol Saline. Jogjakarta : Jurnal Sains Fakultas Kedokteran Hewan Universitas Gajah Mada 24 (1): 65-70.

Arruda, R.P., B.A. Ball, C.G. Gravance. A.R. Garcia and I.K. Liu. ,(2002) Effects of Extenders and Cryoprotectants on Stallion Sperm Head Morphometry. USA : Theriogenology 58: 253-256.

Banaszewska, D., S. Kondracki., A. Wysokinska. ,(2009) Effect of Sperm Concentration on Ejculate for Morpho-metric Trait of Spermatozoas of the Pietrain Breed Boars. Europe : Central Europian Agriculture 10: 383-396.

Gage, M.J.G. and R. Freckleton. ,(2003) Relative Testis Size and Sperm Morphometry Across Mammals: No Evidence For an Association Between Sperm Competition and Sperm Length. USA : Biology Science 270: 625-632.

Hallap, T., M. Haard, U. Jaakma, B. Larsson and H. Rodriguez-Marti



Archive Article

Cover Media Content

Volume : 3 / No. : 1 / Pub. : 2014-04
  1. Morning And Evening Serum Testosterone Level Of Limousine Crossbreed Bulls
  2. Test Potential Polyclonal Antibodies Local (abpo Local Pmsg) And Patents (abpo Patent Pmsg) Pmsg (pregnant Mare Serum Gonadotropin) From Male Rabbit (oryctolagus Cuniculus) To Pregnancy Mice (mus Musculus)
  3. Dna Fragmentation As Apoptotic Indicator And It Relation With Sperm Viability And Motility Of Domba Ekor Gemuk Frozen Semen In Different Thawing Duration
  4. Determination Encoding Genes Heat Stable Toxin (st) And Protein Of Pathogens Escherichia Coli Pili K99 Was Isolated From Dairy Cow Feces Using Polymerase Chain Reaction (pcr)
  5. Biopotensi Pmsg (pregnant Mare Serum Gonadotrophin) Local Horse To Speed Time Arising Estrus, Number Of Follicles, Corpus Luteum, Progesterone Levels And Pregnancy Rate In Madura Cattle
  6. Potential Of Vitamin E (α-tocopherol) Of Malondialdehyde (mda) Levels Testis Mice (mus Muscular) Induced 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (tcdd)
  7. Effect Of “sarang Semut” (myrmecodia Pendans) On The Number Of Spermatogenic Cells In Seminiferous Tubules Of Mice (mus Musculus) With Excessive Physical Treatment
  8. The Effect Of Estrous Syncronization By Pgf2α And Superovulation By Pmsg And Hcg And Artificial Insemination On Heat Response, Pregnancy Rates, Litter Size And Sex Ratio Of The Crossbread Etawa Goat
  9. Examination Of Fresh Semen Quality Of Merino Sheep For Frozen Semen Production In Teaching Farm Of Veterinary Medicine Faculty Of Airlangga University
  10. Identification Of Sperm Morphometry In Merino Sheep And Fat-tailed Sheep
  11. The Correlation Between Body Weight With Body Lenght, Wither’s Height, Heart Girth, Scrotal Circumference, Volume, And Semen Quality Of Peranakan Etawa Goat
  12. Profile Of Progesterone And Insulin-like Growth Factor-i (igf-i) In Pregnant Crossbred Mare Serum
  13. Effect Of Equilibration Time Extender To Post Thawing Motility And Viability Of Merino Sheep’s Sperms In Egg Yolk Skim Diluents
  14. The Effect Of The Combination Progesterone In Sponge Intravaginal With Equine Chorionic Gonadotropin (ecg) To The Occurrence Of Estrous On Fat Tailed Sheep

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