UNIVERSITAS AIRLANGGA



Detail Article

OVOZOA

ISSN 2302-6464

Vol. 2 / No. 1 / Published : 2013-04

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Original Article :

Motility and viability of sheep spermatozoa in skim milk preservation, green beans and a combination of skim milk and green beans

Author :

  1. Suherni Susilowati*1
  2. Yulis Purwitasari*2
  3. Hermin Ratnani*3
  4. Djoko Galiono*4
  1. Departemen Reproduksi Fakultas Kedokteran Hewan Unair
  2. Mahasiswa Fakultas Kedokteran Hewan Unair
  3. Departemen Kedokteran Dasar Fakultas Kedokteran Hewan Unair
  4. Departemen Klinik Veteriner Fakultas Kodokteran Hewan

Abstract :

ABSTRACT This study aims to determine the differences in the percentage of motility and viability of sheep spermatozoa in skim milk cryopreservation, green beans and a combination of skim milk and green beans in 5°C storage. Experimental animals that used were male sheep with fat tail by derivating two ejaculates of semen for three treatments. All three treatments used in these studies is P1 (1ml skim milk + antibiotic + 0,1ml sheep semen), P2 (1ml green beans + antibiotic + 0,1ml sheep semen), P3 (1ml combination of skim milk and green beans with a ratio of 1 : 1 + antibiotic + 0,1ml sheep semen). Furthermore, the data were analyzed using the F test. The results showed that the percentage motility of spermatozoa in skim milk diluent treatment was significantly different (P<0,05) with green beans and thinning treatments were not significantly different (P>0,05) by treatment with a combination of skim milk diluent and green beans. In the treatment of skim milk diluent showed the highest percentage of motility (65,63 ± 10,966) of the three treatments. On the viability percentage of spermatozoa skim milk diluent treatment significantly different (P<0,05) with green beans and thinning treatments were not significantly different (P>0,05) by treatment with a combination of skim milk diluent and green beans. In the treatment of skim milk diluent showed the highest viability percentage (69,67 ± 10,655)  

Keyword :

motility, viability, green beans, skim milk, skim milk and a combination of green beans,


References :

Aboagla, E.M-E and T.Terada. ,(2004) Effect of Egg Yolk During Defreezing Step Of Cryopreser-vation on the Viability of Goat Spermatozoa. Theriogenology 62: 1160-1172. USA : Theriogenology

Park, J. E. and. J. K. Graham,,(1992) Effect of Cryopreservation Proce-dur on Sperm Membrans. Theriogenology 38 : 209-222. USA : Theriogenology

Yildiz, C., A. Kaya., M. Aksoy and T. Tekeli. ,(2000) Influence of sugar supplementation of the extender on motility and acrosomal integrity of dog spermatozoa during freezing. Theriogenology 54: 579-585. USA : Theriogenology





Archive Article

Cover Media Content

Volume : 2 / No. : 1 / Pub. : 2013-04
  1. Fattening Program Using Differencet Types Of Cattle Feed Fermentated Rice Straw And Growth Promoter For Increasing Weight
  2. Effect Of Addition Protein Fertility Associated Antigen (faa) In Semen On Capacitation And Acrosome Reaction Spermatozoa In Bovine Frozen Semen
  3. Motility And Viability Of Sheep Spermatozoa In Skim Milk Preservation, Green Beans And A Combination Of Skim Milk And Green Beans
  4. Productivity, Egg Weight, Egg Yolk Color, And Hatchability Of Egg From Crossbred Mojosari Duck And Alabio Duck
  5. The Effect Of Estrous Syncronization By Pgf2α And Artificial Insemination Using Fresh Semen On Heat Response, Pregnancy Rates, Litter Size And Sex Ratio Of The Crossbread Etawa Goat
  6. Restored Cases Of Repead Breeder P0st Partum In Cattle Using Solution Of Silvers Oligodinamic Ions Intra Uterine

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