UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 8 / No. 3 / Published : 2015-11

Order : 5, and page :271 - 278

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Original Article :

Adaptation capability and antigen stability of newcastle disease virus local isolate on hela cell

Author :

  1. Margaretha Prayudhi Novantiana*1
  2. Rahaju Ernawati*2
  3. Kusnoto*3
  4. Fedik Abdul Rantam*4
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan
  4. Dosen Fakultas Kedokteran Hewan

Abstract :

The aim of this research was to analyze adaptation capability and antigen stability of Newcastle Disease (ND) virus at HeLa cell line. ND virus was inoculated at primary cell CEF and HeLa cell line with two different dose (100μl and 200μl). The virus growth was observed every day to 72 hours and ND virus was inoculated (blind passage) until CPE reached 80% at both kind of culture cell. Two kind of sample, ND virus from both infected culture cell medium and virus from infected cell was collected and prepared for the next step. The measuring of the titer was done by HA micro technique test. Sample with highest titer of HA micro technique test then analyzed by SDS-PAGE and Western Blot method. The result showed that ND virus inoculated at primary culture cell CEF and HeLa were both adapted well, signed by cytophatic effect such as monolayer growth disturbance and the forming of synctia. Meanwhile as the result of ND virus protein identification by SDS PAGE, 4 band was obtained from virus that infected HeLa cell and from ND virus of the culture fluid obtained 12 band. ND virus adapted at HeLa cell by Western blot method showed that ND virus has reactivity against chicken anti ND serum. This proved by 2 protein band formed by virus extraction to HeLa cell and 6 protein band by culture fluid HeLa cell.

Keyword :

Newcastle Disease Virus, HeLa Cell, Cythopathic Effect, SDS-PAGE, Western Blot,


References :

Gallili GE and Ben-Nathan D,(1998) Newcastle disease vaccines 16 (2) : 343–366. : Biotechnology Advances

Kusnoto, S. Subekti, I. K. Sudiana dan S. Koedarto,(2011) Karakterisasi and Isolasi Protein Spesifik Dari Material Excretory-Secretory (ES) Toxocara cati Untuk Pengembangan Diagnostik Toxocariasis dengan Teknik ELISA 13(1): 56-65. : Jurnal Biosains Pascasarjana Universitas Airlangga

Takimoto T, L. Garry, Taylor, C. Hellen, Connaris, J. Susan, Crennell and A. Portner.,(2002) Role of the Hemaglutinin-Neuraminidase Protein in the Mechanism of Paramyxovirus-Cell Membran Fusion 74 (26) : 3028 – 13033 : Journal of Virology





Archive Article

Cover Media Content

Volume : 8 / No. : 3 / Pub. : 2015-11
  1. Genotyping Viral Nervous Necrosis (vnn) In Groupers In East Java And West Nusa Tenggara
  2. Lactoferrin Effect On Biofilm Formation Of Klebsiella Pneumoniae Isolated From Mastitis Cases Of Dairy Cows
  3. Effect Of Insulin Transferrin Selenium (its) Supplementation In Maturation Medium Tcm-199 To Bovine Oocyte Diameter
  4. Supplementation Of Insulin Transferrin Selenium On In Vitro Maturation Cumulus Oocyte Complex (coc) Against To Mitogen Activated Protein Kinase (mapk) Expression And Expanded Cumulus Mass
  5. Adaptation Capability And Antigen Stability Of Newcastle Disease Virus Local Isolate On Hela Cell
  6. Comparison Of Antibody Titre Of Anti Brucella Abortus Using Cft And Ielisa To Cattle With Positive Rbt
  7. Potency Of Apple Vinegar As Alternatif Therapy Against Wound Healing Time Of Staphylococcus Aureus Infection On Mice
  8. Suplementation Of Specific Protein Membrane Spermatozoa In Freezing Medium To Improvement Efforts A Succes Cattle Frozen Semen Quality Post Thawing
  9. Potention Of Alkaloid Sambiloto (andrographis Paniculata L) To Total Leukocytes And Leukocytes Differential Count On Rat After Infected By Salmonella Typhimurium
  10. The Potential Of Outer Membrane Protein Brucella Abortus Local Isolate As Brucellosis Vaccine Development In Indonesia
  11. The Using Of Whole Serum Pmsg (pregnat Mare Serum Gonadotropin) With Sepadex Extracted For Increasing Estrus Induction Of Ettawa Goat
  12. Toxicity Test Of Singawalang (petiveria Alliaceae) Leaves Extract To The Hypertrophy Of Male White Mice (mus Musculus) Cardiac Muscle
  13. Effect Of Benzo(a)pyrene Injection To The Initiation Of Mammae Cancer At Mice

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