UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 4 / No. 3 / Published : 2011-11

Order : 13, and page :225 - 228

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Original Article :

Anti hy preparation as raw material for frozen semen and frozen female embryos

Author :

  1. Husni Anwar*1
  2. Pudji Srianto*2
  3. Wiwiek Misaco Yuniarti*3
  1. Dosen Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

This study was aimed to :1) produce anti HY protein/HY antisera by means of synthesising from the spleen of male rabbit before weaning age,2) define if this HY antibody could be generated by the immunization of adult male rabbit and 3) to examine biologically to define the sex ratio shift on the pups born. Experimental animal used were male rabbit at preweaning age and adult male rabbits subyected to immunization using spleen suspension. Serum was purified using SAS method,followed by dialysis and the level of anti HY antibody was measured using Biuret method. Result of this research showed that from approximately 15 ml serum from week-7 bleeding after the addition of SAS in a ratio of 1:1 which were then homogenized using vortex, finally resulted approximately 4 ml precipitate (semi-solid fom) ,then from BSA standard curve and the absorbance value, it was shown that the level of anti HY antibody was 21825 μg/ml.

Keyword :

HY antigen, HY antibody, Sex determination,


References :

Andonian, L, MB Eslami, AA Muhammadi, J Akbarzadeh, HR Soleimanpour, and DD Fa,(2011) Preparation of HY Antibody in Female Mice as a Model for Sex Pre-selection . : Iranian Journal Publ.Health, Vol. 30,Nos,1-2.

Anzar,M.,He., L., M.M.Buhr, T.G. Kroetsch and K.P. Pauls,(2002) Sperm Apoptosis in Fresh and Cryopreserved Bull Semen Detected by Flow Cytometry and Its Relationship with Fertility . : J. Biol. Reprod. 66, 354-360

Susilawati,T.,(2000) Analisis Membran Spermatozoa Sapi Hasil Filtrasi Sephadex dan Sentri-fugasi Gradien Densitas Percoll pada Proses Seleksi Jenis Kelamin Surabaya : Disertasi Pascasarjana Universitas Airlangga





Archive Article

Cover Media Content

Volume : 4 / No. : 3 / Pub. : 2011-11
  1. Potential Carbofuran Induced Oxidative Stress, Decreased Choline Esterase And Increased Brain Cell Embryonal Death
  2. Crossbreds Entog And Duck Artificial Insemination Technology Of Diluted And Doses For Fertibily
  3. Pengaruh Pemberian Alkaloid Daun Jarong (achyranthes Aspera Linn) Pada Mencit Yang Terkena Kanker Mammae Terhadap Gambaran Hitung Jenis Leukosit
  4. Motilitas, Persentase Hidup Dan Keutuhan Membran Spermatozoa Domba Ekor Gemuk Post Thawing Dalam Tiga Macam Diluter
  5. The Quality Of Ram Sperm After Washing With Brackett And Oliphant’s (bo) Medium At Skim Milk And Egg Yolk Diluent
  6. Hematology Parameter Of Kacang Goats In Mojosarirejo Village Driyorejo Gresik
  7. The Utilization Of Ginger (zingiber Officinale ) As Natural Antibacterial On Pasteurized Milk Based On The Reduce Number Of Escherichia Coli
  8. Difference Optical Density405nm Value Antibody Of Layer Vaccinated With Infectious Bronchitis Active Monovalent And Infectious Bronchitis Activebivalent (ib-nd) By Using Indirect Elisa
  9. The Use Of Recycle Soybean Fermented Cake (tempe) With Cellulolytic Bacteria As Corn Substitution To Consumption Feed, Egg Production And Feed Convertion Ratio Of Layer Duck
  10. The Prevalence Of Helminthiasis Gastrointestinal Tract Of Sumatran Tiger (panthera Tigris Sumatrae) And Bengal Tiger (panthera Tigris Tigris) In Three Different Areas Of Conservation
  11. Supplement Of Acrosin To The Post Thawing Sperm Of Half-breed Of Etawa Goat (pe) Towards Capacitation And Acrosome Reaction Of Spermatozoa
  12. The Effectiveness Of Glutaraldehyde And Didecyl Dimethyl Ammonium Chloride Combination As A Disinfectant To Decrease Total Number Of Bacteria On Layer Chicken Cages
  13. Anti Hy Preparation As Raw Material For Frozen Semen And Frozen Female Embryos
  14. Aphrodisiac Bioscreening Models, Profile Of Justicia Gendarussa Burm.f., Pimpinella Pruatjan Molkenb And Pangium Edule Reinw. Extracts On Isolated Frog Gastric Smooth Muscle
  15. Application Of Human Menopose Gonadotrophin (hmg) Toward Follicle Growth In Cows Ovary Hypofunction

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