UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 2 / No. 1 / Published : 2009-02

Order : 13, and page :67 - 72

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Original Article :

Concentration of total protein from goat seminal plasma by biuret method

Author :

  1. Suherni Susilowati*1
  1. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

Semen is the liquid or semigelatinous cellular suspension containing the male gametes orspermatozoa and secretions from accessory organs of the male reproductive tract. The fluid portion of this suspension, which is formed at ejaculation, is known as seminal plasma. Seminal plasma is composite secretion arising from a number of sources including the testes, epididymis, and accessory glands of the male. Seminal plasma appears to be more important in natural mating of cow where the ejaculate is deposited in the vagina. Seminal plasma contains of citric acid, ergothionine, fructose, glycerylphosphorylcholine and sorbitol. Appreciable quantities of ascorbic acid, amino acids, peptides,proteins, lipids, fatty acids and numerous enzyme. Seminal plasma was reported to be important for maintaining spermatozoa motility, improving sperm viability and increasing the resistance of boar spermatozoa to cold shock damage.Protein of plasma seminalis can be isolated by protein purification with added Phosphat Buffer Saline (PBS) and Phenylmethenesulfonyl fluoride (PMSF) and then centrifugated. The supernatan added by etanol absolut and finally to measure protein total concentration by using Biuret method. The result of this experiment show that the protein total concentration was 8200 ppm/cc plasma seminalis.

Keyword :

goat, seminal plasma, protein, biuret, .,


References :

Aulani’am,(2004) Prinsip Dan Teknik Analisis Biomolekul Malang : Fakultas Pertanian Universitas Brawijaya Press

Beatriz B, Perez-Pe R, Gallego M, Tato A, Osada J, Muino Blanco T and Cebrian-Perez JA.,(2000) Seminal plasma proteins revert the cold shock damage on ram sperm membrane United States : J. Biology Reproduction

Aulani’am,(2004) Dasar-Dasar Biomolekul Malang : Fakultas Pertanian Universitas Brawijaya Press





Archive Article

Cover Media Content

Volume : 2 / No. : 1 / Pub. : 2009-02
  1. The Effect Of Water Extract From Phyllanthus Niruri L. Is Immunostimulator In Newcastle Disease Vaccinated Chicken
  2. Proliferation Activity Of Gland Mammae After Leaves Gynura Procumbens Extract Which Dmba (dimethylbenz(a)antrasen) Initiation On Sprague Dawley Rat
  3. Growth Differentiation Factor-9 (gdf-9) Characterization On Bovine Oocyte Collected From Preanthral Follicle Using Dotblotting Method
  4. Optical Density Value Of Antibody Against Pregnancy-associated Glycoproteins (pags) From The Pregnant Dairy Cow Blood Serum In Male Local Rabbit (oryctolagus Cuniculus)
  5. Implementation Studies Of Artificial Insemination Program Of Beef Cattle In West Java
  6. Glycoprotein Of Human Membrane Spermatozoa
  7. Specifitacy Test Anti-prostaglandin F2a Indirect Elisa Method
  8. The Sensitivity Test Of Some Antimicrobial Agents To Staphylococcus Aureus Of Cause Mastitis At Dairy Cattle At Around Of Suka Makmur Dairy Cooperative Grati Pasuruan
  9. Correlation Between Subclinical Mastitis Stages Use California Mastitis Test With Total Bacteria In Milk
  10. Detection Of Progesterone Of Pregnant Dairy Cattle Using Enzyme Immunoassay (eia) Method
  11. Identification Of Encoding Gene For Fibrinogen Binding Protein On Staphylococcus Aureus In Mastitic Milk
  12. The Polymorphism Of Coagulase Gene As Searching Strain Variability Of Staphylococcus Aureus From Bovine Mastitis Cases
  13. Concentration Of Total Protein From Goat Seminal Plasma By Biuret Method
  14. The Consumption Of Crude Protein Of Haylage Complete Feed On Ongole Crossbreed Steers
  15. Crude Protein Contents And Dry Matter In Ipome Sp Fermentation As Alternative For Feed Stuff
  16. The Effect Of Forced Moulting In The Avian As Causes Of Zoonocis Case Salmonella Enteridis (se)

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