UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 9 / No. 3 / Published : 2016-11

Order : 14, and page :91 - 97

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Original Article :

Expression of cytochrome c and capacitation reaction frozen semen fresian holstein bulls (fh) after thawing in different temperature and duration in diluent milk egg yolk

Author :

  1. Ragil Angga Prastiya*1
  2. Sitti Zainab*2
  3. Hardijanto*3
  1. Dosen Fakultas Kedokteran Hewan
  2. Departemen Kebidanan, high STIKES Surabaya
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The main problems in the frozen semen Holstein Friesian cows are poor qualityof semen after thawing. During the freezing process between 20-80% spermatozoa can bedead. The freezing process can also lead to decreased sperm motility due to the influenceof diluent or damage caused by cold shock. This study examined the motility, viability,capacitation, and the expression of cytochrome C on spermatozoa Fresian Holstein bulls(FH) after thawing at a temperature and time given diluter Milk Egg Yolk (SKT). We use24 frozen semen of dairy cows were divided into 5 treatment. The results showed thatat 37 0C temperature and 30 seconds duration have a number of maximum motility andviability, capacitation figures are not too high, and the expression of cytochrome C is low.The conclusion of this study, thawing can be done at 37 0C temperature and 30 secondsduration because of the quality of spermatozoa produced is still quite good.

Keyword :

Cytochrome C, Capacitation, Milk Egg Yolk (SKT), Thawing, Friesian Holstein,


References :

Arthur, G.H., D.E. Noakes, P. Harrold and T.J. Parkinson. ,(1996) Veterinary Reproduction and Obstetrics. Seventh Edition. pp. 589-595 : W.B. Saunders Company Ltd. London. England.

Chatterjee S., E.R. Smith , K. Hanada, V.L. Stevens and S. Mayor. ,(2001) . GPI Anchoring Leads to Sphingolipid-dependent Retention of Endocytosed Proteins in The Recycling Endosomal Compartment. 20:1583–1592. : EMBO J.

Deshpande, S.S., Angkeow, P., Huang, J., Ozaki, M., and Irani, K. ,(2000) . Rac1 Inhibits TNF-α-Induced Endothelial Cell Apoptosis: Dual Regulation by Reactive Oxygen Species. 14 : 1705-1714. : The FASEB Journal





Archive Article

Cover Media Content

Volume : 9 / No. : 3 / Pub. : 2016-11
  1. The Effect Of Spirulina Platensis Extract Immersion As Immunostimulant To Intestine Histopathology Of Aeromonas Hydrophila Infected Gouramy (osphronemus Gouramy)
  2. Effect Of Vitamin E And Vitamin C Combination As Preventive Measure On The Leydig Cell Number In Mice (mus Musculus) Treated With Borax
  3. The Influence Of Beta Carotene To Live Fetus Percentage In Mice (mus Musculus) That Exposured By Cigarette Smoke
  4. Effects Of Vitamin C And Vitamin E Combinationas Prevention On The Liver Histopathological In Mice (mus Musculus) Treated With Borax
  5. Effect Of Moringa Oleifera Leaf Extract On The Percentage Of Necrotic Purkinje Cells In Mice (mus Musculus) Induced By Methylmercury
  6. Hepatoprotective Effect Of Andrographis Paniculata Ness. Leaves Extract Towards Mice (mus Musculus L.) Liver Histopathology Which Exposured Bylead Acetate Orally
  7. The Effect Of Mimosa Pudica Root Extract To Histopathological Representation Of Rattus Norvegicus Liver Injected With Naja Sputatrix Venom
  8. Kualitas Spermatozoa Domba Merino Pada Sisi Anoda Hasil Pemisahan Dengan Teknik Ess ( Electric Separating Sperm)
  9. Effectivity Of Rat Bone Marrow Mesenchymal Stem Cell Therapy On Rattus Norvegicus Exposed To Carbon Black As Observed From Caspase-3 Expression
  10. Larvaside Effectiveness Of Srikaya Seed Extract (annona Squamosa L.) Against Mortality Of Anopheles Aconitus Larvae
  11. Therapeutic Effect Rat Bone Marrow Mesenchymal Stem Cell (rbm-msc) At The White Rat Endothelial Cells Induced Carbon Black To Total Hofbauer Cells
  12. Identification Of Bacteri Methicillin Resistent Staphylococcus Aureus And Methicillin Sensitive Staphylococcus Aureus From Dog Nasal Mucous
  13. Expression Of Cytochrome C And Capacitation Reaction Frozen Semen Fresian Holstein Bulls (fh) After Thawing In Different Temperature And Duration In Diluent Milk Egg Yolk
  14. Detection Of Bacterial Contamination Amount At Meat Which Is Soaked In Chitosan Solution With Total Plate Count Method
  15. The Effect Of Electrical Stunning On The Ph Values Musculus Fibularis Longus Broiler Chicken

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