ISSN 1979-1305
Vol. 3 / No. 1 / Published : 2010-02
Order : 16, and page :75 - 80
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Original Article :
Identification of pregnancy specific protein b (pspb) from placenta foetalis (cotyledon) freishian holstein cows
Author :
- Abdul Samik*1
- Dosen Fakultas Kedokteran Hewan
Abstract :
This research was aimed to find out a substance that was useful in early pregnancy diagnosis of FreishianHolstein cows. Pregnancy Specific Protein B (PSPB) was isolated, purified and partially characterized from cotyledonFreishian Holstein cows. Characterization of PSPB protein was conducted using SDS-PAGE and Western Blot.Antisera were developed against PSPB and by immunohistochemical techniques the protein was localized to thebinucleated cells of the cotyledon.The estimated molecular size of Freishian Holstein cows PSPB was 59,88 kDa with the concentration of0dP,eS4nP7sB5it4 yp (r( o1OtseDt iwn) ewienek cr aeof t0tye,lr1e b7do9oo n±s w t0ear,0)s 1a60n42d8 20( , b2ne5gf6/om ±rel .0 iP,m4S8mP4B2u n (p i2rzonatdtei owinne )ce;o k1u a,l4fdt6 ei6nr db±uo 0co,es3 ta2enr8)t8.i - (P S3rPdB w aenetikb oafdtye r wimitmh uthneiz vaatiloune )o; f1 o,9p3ti6c a±l
Keyword :
cotyledon, PSPB, anti-PSPB antibody, ., .,
References :
Abbas, K.A., A.H Licthman dan J.S Pober.,(2000) Antibodies and Antigens. Cellular and molecular immunology. 4 th ed. Philadelphia : WB Saunders Co.
Aulani,(2004) Prinsip dan Teknik Analisis Biomolekul Malang : Universitas Brawijaya Press
Archive Article
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Content |
Volume : 3 / No. : 1 / Pub. : 2010-02 |
- Apllication Of Frozen Semen Plus Tyrosine Kinase To Increased Conception Rate Of Beef Cattle
- Isolation, Identification And Characterization Of Inhibin B Protein Of Rattus Norvegicus Sertoli Cell Culture As Male Hormonal Contraception Candidate
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- Viability And Motility Muscovy Drake's (cairina Moschata) Spermatozoa In A Diluter Combination Of Skim Milk, Fructose And Egg Yolk
- Addition Of Growth Differentiation Factor-9 (gdf-9) At Culture Medium In Vitro On Chromosome Profile Of Bovine's Oocyte
- The Potency Of A Black Soybean Milk And Yellow Soybean Milk On The Level Of Triglycerides In Rat (rattus Novergicus) Fed With A High Fat Diet
- The Effect Of Centrifugation On Quality Of Motility, Viability And Acrosom Cap Of Goat Sperm
- Benzopyrene Induce Apoptosis Of The Lung Cells In Ratus Norvegicus
- Partenogenetik Development Of Mouse Oocytes Activation Using Ethanol And 6-dmap By In Vitro
- Identification Of Pregnancy Specific Protein B (pspb) From Placenta Foetalis (cotyledon) Freishian Holstein Cows
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