UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 1 / No. 2 / Published : 2008-06

Order : 8, and page :39 - 42

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Original Article :

Identification of protein growth differentiation factor-9 (gdf-9) isolated from oocytes dominant follicles of bovine ovum

Author :

  1. Widjiati*1
  2. Nur Zahrotul Hayati*2
  3. Ismudiono*3
  4. Sukmanadi*4
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga
  4. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

This research comprises a series of researches on protein Growth Differentiation Factor-9 (GDF-9) fraction in in vitro maturation process to improve the quality of oocytes as oocyte bank for in vitro embryo production. The research is aimed at identification and characterization of protein Growth Differentiation Factor-9 (GDF-9) isolated from oocytes dominant follicles of bovine ovum. Bovine ovary obtained from a slaughterhouse was aspired in its ovary follicles then extracted by sonication technique. Identification of protein Growth Differentiation Factor-9 (GDF-9) fraction was carried out by means of Sodium Dodecyl Sulphonate Polyacrylamide Gel Electrophoresis (SDS-PAGE). From the results of research, several protein fractions were obtained. Based on the calculation of regression equation resulting from protein marker to determine the molecular weight of Growth Differentiation Factor-9 (GDF-9) protein, 8 protein fractions were determined, they are : 146.46 kDa, 116.12 kDa, 101.02 kDa, 51.06 kDa, 39.94 kDa, 34.75 kDa, 31.67kDa, 15.07kDa. Protein appearing in the protein band the molecular weight of 51.06 kDa was identified as Growth Differentiation Factor-9 (GDF-9) protein fraction playing a role in follicle development.

Keyword :

Growth Differentiation Factor-9 (GDF-9), oocyte, dominant follicle, ., .,


References :

Elvin, J. A., Y. Changning, P. Wang., K. Nishimori and M. M. Matzuki,(1999) Molecular Characterization of the follicle defects in the Growth differentiation factor-9 (GDF-9) deficient ovary . : J. Mol. Endo.

Jayawardhana, B. C., S. Takashi, N. Hiromi, K. Etsushi, T. Masafumi and M. Akio,(2006) Hormonal regulation of expression growth differentiation factor-9 (GDF-9) receptor type I and II genes in vitro the bovine ovarian follicle . : J. Repro.





Archive Article

Cover Media Content

Volume : 1 / No. : 2 / Pub. : 2008-06
  1. Prevalence Rate Of Bovine Mastitis From Several Dairy Herds In East Java
  2. The Sambiloto Leaf As An Active Subtance Of Dipping Solution On A Dairy Cattle Mastitis
  3. Protein Characterization Of Insulin Like Growth Factor – I Complex Of Goat Seminal Plasma As An Capacitation Medium Of Sperm
  4. The Influence Of Infected Oocyst Of Eimeria Tenella On Meat Quality Of Broilers
  5. Increasing Yoghurt Flavour Dan Texture From Goats Milk By Adding Inoculan Consentration
  6. The Sensitivity Test Of Several Antimicrobial Agents To Escherichia Coli That Caused Mastitis In Dairy Cows At Around Of Suka Makmur Dairy Cooperative Grati Pasuruan
  7. Sex Determination From Mandible By Angular Morphometric Methods On Goat (capra Spp.)
  8. Identification Of Protein Growth Differentiation Factor-9 (gdf-9) Isolated From Oocytes Dominant Follicles Of Bovine Ovum
  9. Effect Of Teratogenic Insecticide Carbofuran At Chicken Embryo
  10. The Addition Of Heparin And Hypotaurine In Capacitation Medium Earle’s Balanced Salt Solution (ebss) On Embryo Development In Vitro Fertilization Of Dairy Cattle
  11. The Influence Of 60% Ethanol Fraction And Water Fraction Of Justicia Gendarussa Burm.f Leaf On Pegion (columba Livia) Emetic Activity
  12. Biosynthesis And Regulation Of Sex Pheromones (z)-9-tricosene In The Housefly, Musca Domestica
  13. Detection Of Rabbits Serum Antibody Which Were Immunized By Sarcoptes Scabiei Var. Caprae

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